Sonoporation of suspension cells with a single cavitation bubble in a microfluidic confinement
Sonoporation of suspension cells with a single cavitation bubble in a microfluidic confinement
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DOI：
10.1039/b712897p
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Ohl, Claus-Dieter
中科院分区：
文献类型：
--
作者：
Le Gac, Severine;Zwaan, Ed;Ohl, Claus-Dieter
在这里，我们报道了在微流控条件下，使用单个激光诱导的空化气泡对HL60(人早幼粒细胞白血病)悬浮细胞进行超声旋转术。空化气泡可以诱导其附近的细胞发生膜破裂。通过钙黄绿素负载细胞释放钙黄绿素或摄取台盼蓝来检测置于微流体室中的悬浮细胞的膜完整性。远离空化气泡中心四倍R-max(最大气泡半径)的细胞完全不受影响，而距离0.75R-max的细胞发生穿孔的概率为75%。这些结果使我们能够将0.75R-max的距离定义为空化气泡与HL60悬浮细胞的临界相互作用距离。这些实验表明，悬浮细胞的流动诱导穿孔适用于芯片实验室系统，这可能是电穿孔的一种有趣的替代方案。
We report here the sonoporation of HL60 (human promyelocytic leukemia) suspension cells in a microfluidic confinement using a single laser-induced cavitation bubble. Cavitation bubbles can induce membrane poration of cells located in their close vicinity. Membrane integrity of suspension cells placed in a microfluidic chamber is probed through either the calcein release out of calcein-loaded cells or the uptake of trypan blue. Cells that are located farther away than four times R-max (maximum bubble radius) from the cavitation bubble center remain fully unaffected, while cells closer than 0.75 R-max become porated with a probability of >75%. These results enable us to define a distance of 0.75 R-max as a critical interaction distance of the cavitation bubble with HL60 suspension cells. These experiments suggest that flow-induced poration of suspension cells is applicable in lab-on-a-chip systems, and this might be an interesting alternative to electroporation.