Neonatal whisker clipping alters behavior, neuronal structure and neural activity in adult rats
Neonatal whisker clipping alters behavior, neuronal structure and neural activity in adult rats
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DOI：
10.1016/j.bbr.2012.10.022
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发表时间：
2013-02-01
影响因子：
2.7
通讯作者：
Lee, Li-Jen
中科院分区：
文献类型：
--
作者：
Chu, Yu-Fei;Yen, Chen-Tung;Lee, Li-Jen
早期经验在感觉系统的发育过程中起着至关重要的作用。例如，新生儿手术操作啮齿动物的胡须会导致以后生活中神经活动和行为的改变。然而，尽管外科手术损害了感觉通路，但很难研究胡须剥夺对成年动物的影响。为了解决这个问题，我们从出生当天(P0)到出生后第3天(P)进行了一种非侵入性的新生儿胡须修剪(WC0-3)范例，并检查了他们成年时的行为表现。随着胡须的完全再生，WC0-3组大鼠在跨越缺口任务中表现出比对照组更短的可跨越距离，这表明它们的胡须特异性触觉功能存在缺陷。在他们的躯体感觉皮层中，第四层带刺的星状神经元降低了树突的复杂性和棘突的密度。在一个新的环境中探索后，依赖活动的即刻早期基因c-fos在躯体感觉皮质中的表达显著增加。而WC0-3大鼠的c-Fos阳性细胞数明显少于对照组，提示WC0-3大鼠皮层间感觉相关神经活动的传递存在缺陷。综上所述，我们的结果证明了早期触觉经验在桶形皮质中建立层特异性兴奋性连接的作用。早期的感觉不足会在感觉系统中留下长期的功能缺陷。(C)2012爱思唯尔B.V.保留所有权利。
Early experience plays critical roles during the development of sensory systems. For example, neonatal surgical manipulations of the whiskers in rodents lead to altered neural activity and behaviors later in life. However, while surgical procedures damage the sensory pathway; it is hard to examine the impact of whisker deprivation on adult animals. To address this issue, we performed a neonatal whisker clipping (WC0-3) paradigm, a non-invasive procedure, from the day of birth (P0) to postnatal day (P) 3, and examined behavioral performances in their adult age. With fully regrown whiskers, the WC0-3 rats exhibited shorter crossable distance than controls in a gap-crossing task, suggesting a defect in their whisker-specific tactile function. In their somatosensory cortex, the layer IV spiny stellate neurons had reduced dendritic complexity and spine density. After exploration in a novel environment, the expression of an activity-dependent immediate early gene, c-fos, increased dramatically in the somatosensory cortex. However, in WC0-3 rats, the number of c-Fos positive cells was less than those in control rats, indicating a fault in transducing sensory-related neural activity between cortical layers in WC0-3 rats. Together, our results demonstrate the roles of early tactile experience on the establishment of layer-specific excitatory connection in the barrel cortex. Early sensory insufficiency would leave long-lasting functional deficits in the sensory system. (c) 2012 Elsevier B.V. All rights reserved.