Internal Gene Cassette From a Human-Origin H7N9 Influenza Virus Promotes the Pathogenicity of H9N2 Avian Influenza Virus in Mice.
Internal Gene Cassette From a Human-Origin H7N9 Influenza Virus Promotes the Pathogenicity of H9N2 Avian Influenza Virus in Mice.
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人源 H7N9 流感病毒的内部基因盒促进 H9N2 禽流感病毒在小鼠中的致病性
DOI：
10.3389/fmicb.2020.01441
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发表时间：
2020
影响因子：
5.2
通讯作者：
Li Zejun
中科院分区：
文献类型：
--
作者：
Zhang Miaomiao;Zhao Chen;Chen Hongjun;Teng Qiaoyang;Jiang Lang;Feng Daobin;Li Xuesong;Yuan Songhua;Xu Jianqing;Zhang Xiaoyan;Li Zejun
H9 N2禽流感病毒是在家禽中流行最广泛的病毒之一，由于其与其他流感病毒的频繁基因重排，对人类健康构成巨大的潜在威胁。在这项研究中，我们产生了一系列的H9 N2-H7N9重组病毒，并在小鼠模型中检测其致病性。我们发现，HA或HA和NA的组合替换H9 N2背景导致病毒诱导的致病性没有实质性变化，而含有H7N9内部基因的H9 N2病毒与亲本H9 N2病毒相比具有显著更高的毒力。这种增加的致病性与小鼠和MDCK细胞中增强的病毒复制有关。我们进一步证明了来自H7N9病毒的病毒核糖核蛋白复合物具有比来自其H9 N2对应物的更高的活性。总的来说，我们的数据表明，H9 N2和H7N9病毒之间的遗传相容性促进了在家禽中共同传播的H7N9和H9 N2病毒之间的重配，并且内部基因替换将H9 N2病毒转化为对人类健康的新威胁。
H9N2 avian influenza virus is one of the most widely circulating viruses in poultry and poses a huge potential threat to human health due to its frequent gene reassortment with other influenza viruses. In this study, we generated a series of H9N2-H7N9 reassortant viruses and examined their pathogenicity in a mouse model. We found that HA or combined HA and NA replacement on the H9N2 background led to no substantial change in the virus-induced pathogenicity, whereas H9N2 virus containing H7N9 internal genes had significantly higher virulence in comparison to the parental H9N2 virus. This increased pathogenicity is associated with enhanced viral replication both in mice and in MDCK cells. We further demonstrated that the viral ribonucleoprotein complex from H7N9 virus possessed higher activity than that from its H9N2 counterpart. Collectively, our data demonstrated that genetic compatibility between H9N2 and H7N9 viruses facilitated the reassortment between H7N9 and H9N2 viruses co-circulated in poultry and that internal gene replacement would convert H9N2 virus into a novel threat to human health.