Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotoblotic and convectional mice
Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotoblotic and convectional mice
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DOI：
10.1099/jmm.0.46525-0
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发表时间：
2007-03-01
影响因子：
3
通讯作者：
Nicoli, Jacques R.
中科院分区：
文献类型：
--
作者：
Martins, Flaviano S.;Rodrigues, Ana Cristina P.;Nicoli, Jacques R.
作者实验室的先前结果表明，在“cachaqa”生产过程中分离的酿酒酵母菌株905能够在无菌和常规小鼠的胃肠道中定植和存活，并保护这些动物免受肠道沙门氏菌血清型鼠伤寒沙门氏菌或艰难梭菌的口服攻击。本文研究了S. cerevisiae 905对Salm.在gnotobiotic和/或常规小鼠中评价鼠伤寒沙门氏菌（肠系膜淋巴结、派伊尔集合淋巴结、脾、肝）以及对免疫系统（枯否细胞的数量、免疫球蛋白的产生、大肠杆菌B-41的清除）的影响。用酵母处理显著减少Salm的易位。鼠伤寒沙门氏菌对无菌动物的肝脏和对常规小鼠的所有试验器官的影响。酵母单相关小鼠（52.9 +/- 15.7）中肝脏中每100个肝细胞的枯否细胞数量显著高于（P < 0.05）无菌对照组（38.1 +/- 9.0）。可能因此，与无菌小鼠相比，在酵母单相关动物中从血流中清除大肠杆菌B41更有效。与无菌组相比，酵母单菌组小鼠肠内容物中分泌伊加和血清中伊加和IgM水平较高（P < 0.05）。结论是，之前研究中观察到的针对病原菌的保护作用可能是由于S治疗小鼠的局部和全身免疫力的调节。酿酒厂905.
Previous results in the laboratory of the authors showed that Saccharomyces cerevisiae strain 905, isolated during 'cachaqa' production, was able to colonize and survive in the gastrointestinal tract of germ-free and conventional mice, and to protect these animals against oral challenge with Salmonella enterica serotype Typhimurium or Clostridium difficile. In the present work, the effects of S. cerevisiae 905 on the translocation of Salm. Typhimurium (mesenteric lymph nodes, Peyer's patches, spleen, liver) as well as on the immune system (number of Kupffer cells, immunoglobulin production, clearance of Escherichia coli B-41) were evaluated in gnotobiotic and/or conventional mice, The treatment with the yeast reduced significantly the translocation of Salm. Typhimurium to liver in gnotobiotic animals and to all the Organs tested in conventional mice. The number of Kupffer cells per 100 hepatocytes in liver was significantly higher (P < 0.05) in yeast mono-associated mice (52.9 +/- 15.7) than in germ-free controls (38.1 +/- 9.0). Probably as a consequence, clearance of E coli B41 from the bloodstream was more efficient in yeast mono-associated animals when compared to germ-free mice. Higher levels (P < 0.05) of secretory IgA in intestinal content and of IgA and IgM in serum were observed in yeast mono-associated mice when compared to germ-free group. Concluding, the protection against pathogenic bacteria observed in a previous study was probably due to a modulation of both local and systemic immunity of mice treated with S. cerevisiae 905.