CALCIUM‐DEPENDENT ALTERATIONS OF NEUROFILAMENT PROTEINS OF RAT PERIPHERAL NERVE
CALCIUM‐DEPENDENT ALTERATIONS OF NEUROFILAMENT PROTEINS OF RAT PERIPHERAL NERVE
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大鼠周围神经神经丝蛋白的钙依赖性改变
DOI：
10.1111/j.1471-4159.1979.tb04530.x
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发表时间：
1979
影响因子：
4.7
通讯作者：
Steven Micro
中科院分区：
文献类型：
--
作者：
W. Schlaepfer;Steven Micro
切除大鼠周围神经节段，并在加速或延迟钙渗透到组织中的条件下在体外孵育。然后将神经节段在1%SDS、8 M尿素和50 mM二硫代硫酸钠中匀浆，并通过SDS聚丙烯酰胺圆盘凝胶电泳分析提取的蛋白质。还固定并处理了代表性样品，用于电子显微镜检查。
Segments of rat peripheral nerve were excised and incubated in vitro under conditions which hasten or retard the penetration of calcium into tissues. The nerve segments were then homogenized in 1%SDS, 8 M‐urea and 50mM‐dithioerythritol and the extracted proteins were analyzed by SDS polyacrylamide disc gel electrophoresis. Representative samples were also fixed and processed for electron microscopic examinations.