"Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates.
"Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates.
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DOI：
10.1016/j.molcel.2010.07.019
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发表时间：
2010-09-10
期刊：
影响因子：
16
通讯作者：
Darwin KH
中科院分区：
文献类型：
--
作者：
Burns KE;Cerda-Maira FA;Wang T;Li H;Bishai WR;Darwin KH
泛素（Ub）提供了将蛋白质传递到真核蛋白酶体进行破坏所需的识别和特异性。原核泛素样蛋白（Pup）在功能上类似于结核分枝杆菌（Mtb）中的Ub，因为它将蛋白质与结核分枝杆菌蛋白酶体相结合。研究表明，Pup和Ub不具有类似的激活和结合靶蛋白的机制。Dop （deamidase of Pup; Mtb Rv2112c/MT2172）将Pup的羧基末端谷氨酰胺脱酰胺为谷氨酸，为其通过蛋白酶体辅助因子A （PafA）连接靶蛋白做准备。虽然研究已经阐明了Pup与蛋白质的结合，但尚不清楚Pup是否可以以类似于Ub从真核蛋白中解偶联的方式从底物中去除。在这里，我们发现分枝杆菌具有Dop提供的“副pylase”活性。副基酶的发现加强了原核生物和真核生物的Pup和Ub标记系统之间的相似性。
Ubiquitin (Ub) provides the recognition and specificity required to deliver proteins to the eukaryotic proteasome for destruction. Prokaryotic ubiquitin-like protein (Pup) is functionally analogous to Ub in Mycobacterium tuberculosis (Mtb) as it dooms proteins to the Mtb proteasome. Studies suggest that Pup and Ub do not share similar mechanisms of activation and conjugation to target proteins. Dop (deamidase of Pup; Mtb Rv2112c/MT2172) deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA). While studies have shed light on the conjugation of Pup to proteins, it was not known if Pup could be removed from substrates in a manner analogous to the deconjugation of Ub from eukaryotic proteins. Here, we show that Mycobacteria have a “depupylase” activity provided by Dop. The discovery of a depupylase strengthens the parallels between the Pup and Ub tagging systems of prokaryotes and eukaryotes, respectively.
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影响因子：
3.6
作者：
Cerda-Maira FA;Pearce MJ;Fuortes M;Bishai WR;Hubbard SR;Darwin KH
通讯作者：
Darwin KH
影响因子：
56.9
作者：
Darwin, KH;Ehrt, S;Nathan, CF
通讯作者：
Nathan, CF
影响因子：
11.4
作者：
Striebel, Frank;Hunkeler, Moritz;Weber-Ban, Eilika
通讯作者：
Weber-Ban, Eilika
影响因子：
82.9
作者：
Gandotra, Sheetal;Schnappinger, Dirk;Ehrt, Sabine
通讯作者：
Ehrt, Sabine
结核分枝杆菌的核酸泛素样蛋白（PUP）蛋白质组[校正]。
影响因子：
3.7
作者：
Festa RA;McAllister F;Pearce MJ;Mintseris J;Burns KE;Gygi SP;Darwin KH
通讯作者：
Darwin KH