A new Rhodamine B-based 'on-off' chemical sensor with high selectivity and sensitivity toward Fe3+ and its imaging in living cells
A new Rhodamine B-based 'on-off' chemical sensor with high selectivity and sensitivity toward Fe3+ and its imaging in living cells
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一种新型基于罗丹明 B 的“开关”化学传感器，对 Fe3 及其在活细胞中的成像具有高选择性和灵敏度
DOI：
10.1016/j.bmc.2014.06.054
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发表时间：
2014-09-01
影响因子：
3.5
通讯作者：
Pang, Tao
中科院分区：
文献类型：
--
作者：
Bao, Xiaofeng;Shi, Jiaxin;Pang, Tao
设计并合成了一种基于罗丹明B和吡咯共轭物（RBPY）的新型荧光化学传感器。紫外-可见吸收光谱和荧光光谱研究表明，在甲醇/水溶液（3：2，v/v，pH 7.10，HEPES缓冲液，0.1 mM）中，RBPY与Fe ~（3+）形成1：1的络合物，对Fe ~（3+）具有很高的选择性和灵敏度。此外，结果表明，在硫离子存在下，RBPY-Fe 3+复合物的形成是完全可逆的，并且也可以用作S2-的有效传感器。重要的是，荧光显微镜实验进一步证明RBPY可以用作检测人肝（L-02）细胞中Fe 3+的荧光探针。(C)2014爱思唯尔有限公司版权所有。
A new fluorescent chemosensor based on a Rhodamine B and pyrrole conjugate (RBPY) has been designed and synthesized. UV-vis absorption and fluorescence spectroscopic studies show that RBPY exhibits a high selectivity and sensitivity toward Fe3+ among many other metal cations in a MeOH/H2O solution (3:2, v/v, pH 7.10, HEPES buffer, 0.1 mM) by forming a 1:1 complex with Fe3+. Furthermore, results reveal that the formation of the RBPY-Fe3+ complex is fully reversible in the presence of sulfide anions and could also be used as an efficient sensor for S2-. Importantly, fluorescence microscopy experiments further demonstrated that RBPY can be utilized as a fluorescent probe for the detection of Fe3+ in human liver (L-02) cells. (C) 2014 Elsevier Ltd. All rights reserved.