Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease, a member of the thermolysin family
Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease, a member of the thermolysin family
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DOI：
10.1128/iai.66.10.4851-4855.1998
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发表时间：
1998-10-01
影响因子：
3.1
通讯作者：
Shinoda, S
中科院分区：
文献类型：
--
作者：
Miyoshi, S;Nakazawa, H;Shinoda, S
创伤弧菌是一种机会性的人类病原体，会引起伤口感染和败血症，其特征是皮肤出血和水肿性损害。这种人类病原体分泌一种金属蛋白酶(V，创伤蛋白酶[VVP])，作为重要的毒力决定因素。皮肤背部皮内注射包括VVP在内的多种细菌金属蛋白酶时，VVP的出血活性最强。重组基底膜凝胶中细菌金属蛋白水解酶的体内出血活性与体外蛋白分解活性显著相关。在两种主要的基底膜成分(层粘连蛋白和IV型胶原)中，只有IV型胶原容易被VVP消化，另外，抗IV型胶原的免疫球蛋白G抗体对VVP引起的出血反应有足够的保护作用，毛细血管的稳定是通过血管内皮细胞的基底面与基底膜的结合来实现的。因此，TV型胶原的特异性降解可能会导致基底膜的破坏，毛细血管的破坏，以及包括红细胞在内的血液成分的泄漏。
Vibrio vulnificus is an opportunistic human pathogen causing wound infections and septicemia, characterized by hemorrhagic and edematous damage to the skin. This human pathogen secretes a metalloprotease (V, vulnificus protease [VVP]) as an important virulence determinant. When several bacterial metalloproteases including VVP were injected intradermally into dorsal skin, VVP showed the greatest hemorrhagic activity. The level of the in vivo hemorrhagic activity of the bacterial metalloproteases was significantly correlated with that of the in vitro proteolytic activity for the reconstituted basement membrane gel. Of two major basement membrane components (laminin and type IV collagen), only type IV collagen was easily digested by VVP, Additionally, the immunoglobulin G antibody against type IV collagen, but not against laminin, showed sufficient protection against the hemorrhagic reaction caused by VVP, Capillary vessels are known to be stabilized by binding of the basal surface of vascular endothelial cells to the basement membrane. Therefore, specific degradation of type TV collagen may cause destruction of the basement membrane, breakdown of capillary vessels, and leakage of blood components including erythrocytes.