Characterization of trypsin-treated forms of the estrogen receptor from rat and lamb uterus.
Characterization of trypsin-treated forms of the estrogen receptor from rat and lamb uterus.
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DOI：
10.1021/bi00638a025
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发表时间：
1977-09
期刊：
影响因子：
2.9
通讯作者：
K. Carlson;L. H. Sun;J. Katzenellenbogen
中科院分区：
文献类型：
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作者：
K. Carlson;L. H. Sun;J. Katzenellenbogen
凯瑟琳·E作者：Carlson，Lee-Hwei K. Sun，and John A.摘要：子宫雌激素受体的分子特性，经过有限的蛋白水解，已经得到了表征。来自羔羊和大鼠子宫的细胞质雌激素受体的轻度胰蛋白酶消化引起受体的显著解聚，产生较低分子量的形式，其配体结合特性保持不变。这些胰蛋白酶化的受体几乎没有再聚集的倾向，因此适合于进一步纯化和详细表征。用蔗糖梯度离心法测得羊和大鼠胰蛋白酶化受体的沉降系数分别为3.04± 0.04S和4.01± 0.09S，用凝胶分配色谱法测得羊和大鼠子宫受体的Stokes半径分别为2.76± 0.23nm和2.94± 0.25nm。在两个系统（pH 7.8和10.2）中的不连续聚丙烯酰胺凝胶电泳显示胰蛋白酶化的羔羊受体为三种不同的组分（两种主要组分和一种次要组分）;大鼠受体稍微分散，但在大鼠受体中的分布不均匀。
Kathryn E. Carlson, Lee-Hwei K. Sun, and John A. Katzenellenbogen* abstract: The molecular properties of uterine estrogen re-ceptors, following limited proteolysis, have been characterized. Mild trypsinization of cytoplasmic estrogen receptor prepa-rations from lamb and rat uterus causes a marked disaggre-gation of the receptor, giving lower molecular weight forms that retain unimpaired their ligand binding properties. These trypsinized receptorshave little tendency to reaggregate and thus are amenable to further purification and to detailed characterization. Sedimentation coefficients obtained by sucrose gradient centrifugation are 3.04±0.04 S for the lamb and 4.01±0.09 S for the rat trypsinized receptors; Stokes radii, determined bygel partition chromatography, are 2.76±0.23 nm for lamb and 2.94±0.25 nm for rat uterine re-ceptor. Discontinuous polyacrylamide gel electrophoresis in two systems (pH 7.8 and 10.2) shows the trypsinized lamb receptor as three distinct components (two major and one minor); the rat receptor is somewhat more disperse, but ap-