Cten mRNA expression was correlated with tumor progression in lung cancers
Cten mRNA expression was correlated with tumor progression in lung cancers
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DOI：
10.1016/s0169-5002(03)00037-0
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发表时间：
2003-05-01
期刊：
影响因子：
5.3
通讯作者：
Fujii, Y
中科院分区：
文献类型：
--
作者：
Sasaki, H;Moriyama, S;Fujii, Y
Cten是最近分离的一个基因，它与张力蛋白具有同源性，表明它是一种粘着斑分子。张力蛋白家族蛋白在细胞运动中发挥重要作用。我们试图确定 cten 表达对接受手术的肺癌患者临床病理特征的影响。使用LightCycler通过逆转录聚合酶链反应（RT-PCR）评估89例肺癌和邻近组织学正常肺样本中cten信使RNA的表达：肺癌组织（1.479+/-2.060）和正常肺组织（1.528+/-1.592，P = 0.8267）之间Cten/3-磷酸甘油脱氢酶（GAPDH）mRNA表达没有显着差异。 cten/GAPDH 表达与年龄、性别或 N 状态之间没有关系。然而，II-IV期肺癌中cten/GAPDH表达的肿瘤/正常比（T/N比）（3.113+/-6.493）显着高于I期肺癌（1.237+/-1.820，P = 0.0316）。 T4肺癌中cten/GAPDH表达的T/N比（4.612+/-9.726）分别显着高于T1肺癌（0.896+/-0.860，P=0.0252）和T2肺癌（1.636+/-2.066，P=0.0470）。因此，cten/GAPDH mRNA 表达与肺癌 T 期和总体分期方面的肿瘤进展证据相关。另外，细胞运动或迁移可能在肺癌的进展中发挥作用。 (C) 2003 Elsevier Science Ireland Ltd. 保留所有权利。
Cten is a recently isolated gene, which has homology with tensin suggesting that it is a focal adhesion molecule. Tensin family proteins play an important role in cell motility. We attempted to determine the influence of cten expression on clinicopathological features in patients with lung cancer who had undergone surgery. Expression of cten messenger RNA was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) in 89 lung carcinomas and adjacent histological normal lung samples using LightCycler: Cten/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression was not significantly different between lung cancer tissue (1.479+/-2.060)and normal lung tissue (1.528+/-1.592, P = 0.8267). There was no relationship between cten/GAPDH expression and age, gender or N-status. However, tumor/normal ratio (T/N ratio) of cten/GAPDH expression was significantly higher in stage II-IV lung cancer (3.113+/-6.493) when compared with stage I lung cancer (1.237+/-1.820, P = 0.0316). T/N ratio of cten/GAPDH expression was significantly higher in T4 lung cancer (4.612+/-9.726) when compared with T1 lung cancer (0.896+/-0.860, P = 0.0252), and T2 lung cancer (1.636+/-2.066, P = 0.0470), respectively. Thus cten/GAPDH mRNA expression has been correlated with evidence of tumor progression in terms of T and overall stage of lung cancer. Alternatively, cell motility or migration might play a role in progression of lung cancer. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.