EFFECT OF FURFURAL AND ETHANOL ON THE GROWTH AND ENERGETICS OF YEAST UNDER MICROAEROBIC CONDITIONS
EFFECT OF FURFURAL AND ETHANOL ON THE GROWTH AND ENERGETICS OF YEAST UNDER MICROAEROBIC CONDITIONS
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DOI：
10.1111/j.1749-6632.1986.tb26517.x
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发表时间：
1986-05-22
影响因子：
5.2
通讯作者：
MUTHARASAN, R
中科院分区：
文献类型：
--
作者：
FIREOVED, RL;MUTHARASAN, R
纤维素材料的酶促转化的替代途径是生物质的酸催化水解;然而，酸水解的一个缺点是产生副产物，其中一些抑制酵母和其它微生物的生长和醇生产。因此，重要的是量化酸水解副产物对乙醇发酵的影响。纤维素酸水解的主要副产物包括糠醛、羟甲基糠醛、甲酸和乙酰丙酸。在本文中，化学计量模型的基础上存在于酵母的生化途径的知识提出。该模型将酵母的宏观变量与其生长和能量学联系起来，从而可以研究生长抑制剂对能量参数的影响。该模型与组分平衡法非常相似，并以类似于Pirt公式的方法将微生物能量学与酵母培养物的宏观行为联系起来。该模型是简单的，但允许量化的实验结果，使发生的“非耦合增长”可以辨别。此外，将微生物的活性或生长与生物学上有用的能量形式ATP相关联是直观和有利的概念，特别是因为重要代谢转化的化学计量约束被并入模型中。虽然正在尝试开发一个动态模型的基础上，本方法，在本文中，我们认为稳态应用的模型和生长抑制剂，如乙醇和糠醛的生长能量的影响进行评估。后一个主题特别重要，因为最近有许多报告声称在生长抑制剂存在下生长参数可能受损。
An alternative route to the enzymatic conversion of cellulosic materials is the acid-catalyzed hydrolysis of biomass; however, one disadvantage of acid hydrolysis is the production of by-products, some of which inhibit growth and alcohol production of yeasts and other microorganisms. Consequently, it is important to quantify the effects of acid hydrolysis by-products on ethanol fermentation. The major by-products of acid hydrolysis of cellulosics include furfural, hydroxymethyl furfural, formic acid, and levulinic acid.’.*In this paper, a stoichiometric model based on the knowledge of biochemical pathways present in yeasts is proposed. This model relates the macroscopic variables of yeasts to their growth and energetics so that the effect of growth inhibitors on the energetic parameters can be studied. The model closely resembles the component balance approach’and relates the microbial energetics to the macroscopic behavior of yeast culture by a method analogous to Pirt’s form~ lation.~ The model is simple and yet allows quantification of the experimental results so that occurrences of “uncoupled growth” can be discerned. Furthermore, relating the activity or growth of a microorganism to a biologically useful form of energy, ATP, is an intuitive and expedient concept, particularly because the stoichiometric constraints of important metabolic transformations are incorporated into the model. While attempts are being made to develop a dynamic model based on the present approach, in this paper we consider the steady-state application of the model and evaluate the effects of growth inhibitors such as ethanol and furfural on the energetics of growth. The latter theme is of particular importance as there have been many reports in the recent past claiming the possibility of impaired growth parameters in the presence of growth inhibitors.’