Simultaneous Determination of Three Aconitum Alkaloids in Six Herbal Medicines by High-Performance Liquid Chromatography
Simultaneous Determination of Three Aconitum Alkaloids in Six Herbal Medicines by High-Performance Liquid Chromatography
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高效液相色谱法同时测定六种中药材中的三种乌头生物碱
DOI：
10.1093/chromsci/48.10.860
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发表时间：
2010-11-01
影响因子：
1.3
通讯作者：
Zhang, Zhi-Qi
中科院分区：
文献类型：
--
作者：
Kang, Xue-Qin;Fan, Zhi-Chao;Zhang, Zhi-Qi
为了同时测定乌头属6种药材中乌头碱、新乌头碱和次乌头碱的含量，对乌头总生物碱的提取条件进行了优化，建立了一种简便的反相高效液相色谱分析方法。总生物碱的萃取率。用酸性乙醇溶液(乙醇：pH 3.0，HAc=85：15，v/v)重复提取3次，得率为98.3%。色谱柱为Phenomeex露娜C18色谱柱，流动相为0.03mol/mL碳酸氢铵(pH=9.50)-乙腈，流速为1.0mL/min。3种生物碱在1.0~200.0µg/mL浓度范围内均呈良好的线性关系(r>；0.999)。平均回收率为96.6~103.1%，检出限和检出限分别为25~37 ng/mL和9~12 ng/mL。定量结果表明，生附子中3种生物碱的含量差异较大，应重视含附子中药材的质量控制。
To simultaneously determine three components of aconitine, mesaconitine, and hypaconitine in six species ofAconitumgenus, an extraction condition for the total alkaloids was specifically optimized and a simple analytical method of reversed-phased highperformance liquid chromatography (HPLC) was developed. The extraction rate of total alkaloids inA. szechenyianumGay was 98.3% for repeated extracting three times with an acidic alcohol solution (alcohol: pH 3.0 HAc = 85:15, v/v). The chromatography was carried out on a Phenomenex Luna C18column by gradient elution with a mobile phase of 0.03 mol/mL ammonium bicarbonate (pH = 9.50)-acetonitrile at a flow rate of 1.0 mL/min. The method for all three alkaloids had good linear relationships (r> 0.999) in the concentration range of 1.0–200.0 µg/mL. The average recoveries were 96.6–103.1%, and the LOQ and LOD were in the range of 25–37 ng/mL and 9–12 ng/mL, respectively. The quantitative results indicated that contents of the three alkaloids varied significantly among crude aconite roots, so quality control of traditional Chinese medicines containing aconite roots should be taken into account.