Determination of the antenna heterogeneity of Photosystem II by direct simultaneous fitting of several fluorescence rise curves measured with DCMU at different light intensities
Determination of the antenna heterogeneity of Photosystem II by direct simultaneous fitting of several fluorescence rise curves measured with DCMU at different light intensities
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DOI：
10.1023/a:1012973402023
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发表时间：
2001-01-01
影响因子：
3.7
通讯作者：
Naus, J
中科院分区：
文献类型：
--
作者：
Lazár, D;Tomek, P;Naus, J
到目前为止，已经发展了几种从DCMU测量的荧光上升曲线来确定光系统II的天线异质性的方法。利用这些方法，根据天线的异质性确定了两种、三种或四种类型的光系统II。然而，其中一些方法的准确性仍在争论中。在这里，我们提出了一种新的方法来确定光系统II的天线异质性，该方法是基于直接同时拟合用DCMU在不同光激发强度下测量的几条荧光上升曲线。由于用同一模型同时拟合出在不同光照条件下测得的多条曲线，提高了模型参数确定的可靠性和准确性。我们的方法被应用于两种不同结构的类囊体膜材料：小麦叶片和绿藻衣藻细胞。
Several methods for determination of the antenna heterogeneity of Photosystem II from fluorescence rise curves measured with DCMU have been developed so far. Using these methods, two, three or four types of Photosystem II with respect to the antenna heterogeneity were determined. However, the accuracy of some of these methods is under debate. Here, we present a new method for the determination of the antenna heterogeneity of Photosystem II. The method is based on direct simultaneous fitting of several fluorescence rise curves measured with DCMU at different intensities of light excitation. As several curves measured under different light conditions are fitted simultaneously by the same model, reliability and accuracy in determination of model parameters increase. Our method was applied to two plant materials with different structure of the thylakoid membrane: wheat leaves and cells of green alga Chlamydomonas reinhardtii.