Development of Nonheme {FeNO}7 Complexes Based on the Pyrococcus furiosus Rubredoxin for Red-Light-Controllable Nitric Oxide Release
Development of Nonheme {FeNO}7 Complexes Based on the Pyrococcus furiosus Rubredoxin for Red-Light-Controllable Nitric Oxide Release
复制标题
基于激烈火球菌红红还原蛋白的非血红素 {FeNO}(7) 复合物的开发，用于红光可控一氧化氮释放
DOI：
10.1021/acs.inorgchem.1c02089
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发表时间：
2021-09-10
影响因子：
4.6
通讯作者：
He，Chunmao
中科院分区：
文献类型：
--
作者：
Lin，Shaomin;He，Chunmao
一氧化氮(NO)是一种重要的生物信使，在多种生理过程中发挥着重要作用。光可控NO释放剂因其作为NO相关研究和治疗试剂的潜力而引起人们的极大兴趣。在这里，我们通过在重新设计的铁-硫蛋白支架中构建一个非血红素{FeNO}7中心，开发了一对红光可控的NO释放子pfRD-C9A-{FeNO}7和pfRD-C42A-{FeNO}7(pfRD=金黄色葡萄球菌)。电子顺磁共振自旋捕捉和Griess分析证实，这些配合物在空气和热稳定性的同时，对红光具有很高的灵敏度和时间精度。DNA裂解实验也证实了这些复合体释放时间受控的NO。总体而言，这项研究表明，这种基于蛋白质的非血红素亚硝酸铁系统可能是一种可行的化学工具，用于精确地给药NO。
Nitric oxide (NO) is an essential biological messenger, contributing a significant role in a diverse range of physiological processes. The light-controllable NO releasers are of great interest because of their potential as agents for NO-related research and therapeutics. Herein, we developed a pair of red-light-controllable NO releasers,pfRd-C9A-{FeNO}7andpfRd-C42A-{FeNO}7(pfRd =Pyrococcus furiosusrubredoxin), by constructing a nonheme {FeNO}7center within the redesigned iron–sulfur protein scaffolds. While shown to be both air and thermally stable, these complexes are highly sensitive to red-light irradiation with temporal precision, which was confirmed by electron paramagnetic resonance spin trapping and Griess assay. The temporally controlled NO release from these complexes was also demonstrated in DNA cleavage assay. Overall, this study demonstrates that such a protein-based nonheme iron nitrosyl system could be a viable chemical tool for precise NO administration.