Root and bacterial secretions regulate the interaction between plants and PGPR leading to distinct plant growth promotion effects
Root and bacterial secretions regulate the interaction between plants and PGPR leading to distinct plant growth promotion effects
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DOI：
10.1007/s11104-015-2743-7
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发表时间：
2016-04
期刊：
影响因子：
4.9
通讯作者：
Dongmei Zhou;Xing-Feng Huang;J. Chaparro;D. Badri;D. Manter;J. Vivanco;Jianhua Guo
中科院分区：
文献类型：
--
作者：
Dongmei Zhou;Xing-Feng Huang;J. Chaparro;D. Badri;D. Manter;J. Vivanco;Jianhua Guo
植物根际促生菌（PGPR）因其能够影响宿主植物的生长和产量而在农业中引起了人们的兴趣。ATP结合盒（ABC）转运蛋白通过调节植物根系分泌物在植物-微生物相互作用中发挥重要作用。为了更准确地了解PGPR与寄主植物相互作用的机制和特异性，本研究以PGPR菌株Bacillus cereusAR 156为材料，研究了PGPR菌株与拟南芥野生型（Col-0）及其ABC转运蛋白突变体之间的相互作用对植物生长的影响。cereusAR 156促进了Col-0和Atabcg 30的新梢生长，但抑制了Atabcc 5的生长。细菌挥发物和分泌物促进了Col-0和Atabcg 30的芽生长，但对Atabcc 5没有影响。我们还发现Col-0根系分泌物诱导B. cereusAR 156基因与铁载体和几丁质酶的产生有关，Atabcc 5根系分泌物抑制了这些基因的表达。对Atabcc 5根系分泌物的分析表明，Atabcc 5根系分泌物中氨基酸、有机酸和糖的含量显著低于Col-0。
Background and aimsPlant growth-promoting rhizobacteria (PGPR) have garnered interest in agriculture due to their ability to influence the growth and production of host plants. ATP-binding cassette (ABC) transporters play important roles in plant-microbe interactions by modulating plant root exudation. The present study aimed to provide a more precise understanding of the mechanism and specificity of the interaction between PGPR and host plants.MethodsIn the present study, the effects of interactions between a PGPR strain,Bacillus cereusAR156, andArabidopsis thalianawild type (Col-0) or its ABC transporter mutants on plant growth have been studied.ResultsB. cereusAR156 promoted the shoot growth of Col-0 andAtabcg30but repressed the growth ofAtabcc5. Bacterial volatiles and secretion promoted the shoot growth of Col-0 andAtabcg30 but had no effect onAtabcc5. We also found that root exudates of Col-0 induced the expression ofB. cereusAR156 genes related to siderophore and chitinase production; while root exudates ofAtabcc5inhibited the expression level of those genes. Further analysis of root exudates revealed that amino acids, organic acids, and sugars were significantly less abundant inAtabcc5when compared to Col-0.ConclusionsOur findings highlight that both host plant and PGPR play active roles in the outcome of the plant-microbe interaction.