Study on the fingerprint profile of Monascus products with HPLC-FD, PAD and MS
Study on the fingerprint profile of Monascus products with HPLC-FD, PAD and MS
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DOI：
10.1016/j.foodchem.2008.07.105
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发表时间：
2009-03-15
期刊：
影响因子：
8.8
通讯作者：
Guo, Yanghao
中科院分区：
文献类型：
--
作者：
Zheng, Yunquan;Xin, Yawen;Guo, Yanghao
红曲产品已广泛用作食品添加剂和药品。橘霉素是红曲发酵过程中产生的有毒代谢物。本工作研究了一种超声波处理快速提取方法，以从真菌细胞中完全提取柑橘素。此外，所提出的HPLC方法的优点是无需样品预处理即可同时测定红曲色素和柑橘素。在优化的 HPLC 条件下，实现了色素、橘霉素和其他分解代谢物的基线分辨率。荧光检测器(FD)对柑橘素的检测限达到0.5 ng mL(-1) (S/N > 3/1)。使用光电二极管阵列检测器 (PAD) 检测到八种红曲色素，其中两种尚未描述。八种红曲色素和橘霉素的 HPLC 指纹图谱对于红曲产品的质量控制具有重要意义。 (c) 2008 Elsevier Ltd. 保留所有权利。
Monascus products have been widely used as food additives and pharmaceuticals. Citrinin is a toxic metabolite produced during the Monascus fermentation. In this work, a quick extraction method with ultrasonic treatment was Studied for a complete extraction of citrinin from the fungi cells. Furthermore, the proposed HPLC method has the advantage of the simultaneous determination of Monascus pigments and citrinin without sample pretreatment. Under the optimized HPLC conditions, a baseline resolution of pigments, citrinin and other catabolites was achieved. The detection limit of citrinin reached 0.5 ng mL(-1) (S/N > 3/1) with a fluorescence detector (FD). Eight Monascus pigments were detected, with photodiode array detector (PAD), two of which not yet described. The HPLC fingerprint profile of the eight Monascus pigments and citrinin appears significant for the quality control of the Monascus product. (c) 2008 Elsevier Ltd. All rights reserved.