A Klebsiella pneumoniae strain that shares a type-specific antigen with Shigella flexneri serotype 6 - Characterization of the strain and structural studies of the O-antigenic polysaccharide
A Klebsiella pneumoniae strain that shares a type-specific antigen with Shigella flexneri serotype 6 - Characterization of the strain and structural studies of the O-antigenic polysaccharide
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DOI：
10.1111/j.1432-1033.1996.0786p.x
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发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
Widmalm, G
中科院分区：
文献类型：
--
作者：
Ansaruzzaman, M;Albert, MJ;Widmalm, G
一株肺炎克雷伯氏菌被发现作为唯一的分离与致病潜力的粪便的2岁患者腹泻。与福氏志贺菌血清6型有较强的血清学交叉反应性。交叉反应的抗原被证明存在于细胞壁脂多糖中。对克雷伯氏菌属菌株的致病潜力的研究表明HEp-2细胞的侵袭水平低。然而，对HEp-2细胞的粘附试验以及毒素产生试验均为阴性。该菌株有几个小质粒，并具有多重耐药。这些数据不足以构成估计该微生物致病潜力的基础。未检出K抗原。对克雷伯氏菌O-抗原多糖的结构进行了分析。使用甲基化分析、NMR光谱和Smith降解作为主要方法研究肺炎杆菌菌株。O-抗原多糖具有以下五糖重复单元：-α-L-Rhap-（1- 3）-α-L-Rhap-（1- 2）-α-L-Rhap-（1 - 2）-α-L-Rhap-（1 - 2）-α-L-Rhap-（1- 2）-α-L-Rhap-（1- 2）。肺炎。与福氏志贺菌血清型6 O-抗原的强血清学交叉反应性最可能归因于存在于该血清型的O-多糖重复单元中的结构元件α-L-Rhap-（1->2）-α-L-Rhap。产生抗K. pneumoniae菌株也能凝集S.痢疾血清型1和所有不同血清型的痢疾杆菌菌株。弗莱克斯内里。志贺氏菌菌株在其O-抗原多糖中含有结构元件α-L-Rhap-（1->3）-α-L-Rhap，这可能是观察到的交叉反应性的原因。
A strain of Klebsiella pneumoniae was found as the only isolate with pathogenic potential from the stool of a two-year old patient with diarrhoea. A strong serological cross-reactivity with Shigella flexneri serotype 6 was demonstrated. The cross-reacting antigens were shown to reside in the cell wall lipopolysaccharide. Studies of the pathogenic potential of the Klebsiella strain showed low level of invasion of HEp-2 cells. However, tests for adherence to HEp-2 cells as well as tests for toxin production were negative. The strain had several small plasmids and was multidrug resistant. These data do not form a sufficient basis for estimating the pathogenic potential of the organism. No K antigen was detected. The structure of the O-antigenic polysaccharide from the K. pneumoniae strain was investigated using methylation analysis, NMR spectroscopy, and Smith degradation as the principal methods. The O-antigenic polysaccharide has the following pentasaccharide repeating unit:-->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->.This structure is not identical to any of the previously described O-antigens of K. pneumoniae. The strong serological cross-reactivity with the Shigella flexneri serotype 6 O-antigen can most likely be attributed to the structural element alpha-L-Rhap-(1-->2)-alpha-L-Rhap present in the O-polysaccharide repeating unit of this serotype. Antiserum raised against the K. pneumoniae strain also agglutinated S. dysenteriae serotype 1 and strains of all different serotypes of S. flexneri. The Shigella strains contain the structural element alpha-L-Rhap-(1-->3)-alpha-L-Rhap in their O-antigen polysaccharides which may be responsible for the observed cross-reactivity.