DNA damage levels in prostate cancer cases and controls
DNA damage levels in prostate cancer cases and controls
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DOI：
10.1093/carcin/bgi288
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发表时间：
2006-06-01
期刊：
影响因子：
4.7
通讯作者：
Hu, Jennifer J.
中科院分区：
文献类型：
--
作者：
Lockett, Kristin L.;Hall, M. Craig;Hu, Jennifer J.
这项研究使用碱性彗星试验来评估基础或H2 O2诱导的DNA损伤是否与前列腺癌（CaP）风险相关。使用从158例CaP病例和128例对照中收集的淋巴细胞样本，在一项正在进行的病例对照研究中，我们的结果表明，基础DNA损伤在病例和对照之间没有差异。然而，H2 O2诱导的DNA损伤水平在偶发病例中（平均值+/- SD; 6.61 +/- 4.43，n = 102）显著高于对照组（5.30 +/- 3.60，n = 128）或流行病例（4.47 +/- 3.19; n = 56）。有吸烟史的患者H2 O2诱导的DNA损伤显著高于从不吸烟者（7.57 +/- 4.82 vs 4.52 +/- 2.40; P < 0.001）。校正年龄、种族、良性前列腺增生（BPH）、吸烟史和家族史（FH）后，中位H2 O2诱导的DNA损伤与CaP风险增加1.61倍相关[95%置信区间（CI）= 0.92-2.81]。以H2 O2诱导的DNA损伤的最低四分位数为参照组，第25、50和75四分位数的校正OR为0.90（95% CI = 0.39-2.05）、1.06（95% CI = 0.48-2.35）和2.05（95% CI = 0.96-4.37）（P = 0.046，线性趋势检验）。年龄、吸烟史、家族史和体重指数改变了CaP和DNA损伤之间的关联。我们的研究结果表明，DNA损伤可能与CaP风险有关。然而，需要进行更大规模的病例对照和随访研究，以进一步评估碱性彗星试验在CaP风险评估和预防中的潜在应用。
This study used the alkaline Comet assay to evaluate whether basal or H2O2-induced DNA damage is associated with prostate cancer (CaP) risk. Using lymphocyte samples from 158 CaP cases and 128 controls, collected in an ongoing case-control study, our results showed that basal DNA damage did not differ between cases and controls. However, the H2O2-induced DNA damage level was significantly higher in incident cases (mean +/- SD; 6.61 +/- 4.43, n = 102) than controls (5.30 +/- 3.60, n = 128) or prevalent cases (4.47 +/- 3.19; n = 56). Incident cases with a positive smoking history had significantly higher H2O2-induced DNA damage than never-smokers (7.57 +/- 4.82 versus 4.52 +/- 2.40; P < 0.001). Above-median H2O2-induced DNA damage was associated with a 1.61-fold increase in CaP risk [95% confidence interval (CI) = 0.92-2.81], after adjustment for age, race, benign prostatic hyperplasia (BPH), smoking history and family history (FH). Using the lowest quartile of H2O2-induced DNA damage as the referent group, the adjusted ORs for the 25th, 50th and 75th quartiles were 0.90 (95% CI = 0.39-2.05), 1.06 (95% CI = 0.48-2.35) and 2.05 (95% CI = 0.96-4.37), respectively (P = 0.046, test for linear trend). The association between CaP and DNA damage was modified by age, smoking history, family history and body mass index. Our results suggest that DNA damage may be associated with CaP risk. However, larger case-control and follow-up studies are warranted to further evaluate the potential application of the alkaline Comet assay in CaP risk assessment and prevention.