Exploring Phenotypes for Disease Resilience in Pigs Using Complete Blood Count Data From a Natural Disease Challenge Model
Exploring Phenotypes for Disease Resilience in Pigs Using Complete Blood Count Data From a Natural Disease Challenge Model
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利用来自自然疾病攻击模型的全血细胞计数数据探索猪疾病抵抗力的表型
DOI：
10.3389/fgene.2020.00216
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发表时间：
2020-03-13
影响因子：
3.7
通讯作者：
Plastow, Graham S.
中科院分区：
文献类型：
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作者：
Bai, Xuechun;Putz, Austin M.;Plastow, Graham S.
抗病能力是帮助控制牲畜传染病的一个重要特征。预计疾病抵抗力的提高将持续提高生产效率，因为有抵抗力的动物在面对感染时仍能保持其表现。本研究的目的是利用来自断奶至育肥自然疾病挑战模型的全血细胞计数 (CBC) 数据来识别与疾病恢复力相关的表型，该模型是为了模拟生猪生产商业水平上许多常见病原体引起的疾病压力而建立的。总共 2433 只经过自然疾病挑战模型的 F1 杂交（长白 x 约克夏）阉公猪根据它们在生长和个体治疗方面的不同反应被分为四组（弹性组、普通组、易感组和死亡组）。在攻击前2周、攻击后2周和6周抽取三组用于CBC分析的血液样本：分别是血液1、血液3和血液4。攻击前从健康猪采集的血液 1 的 CBC 未显示组间差异。然而，恢复力强的动物被发现能够启动更快的适应性免疫反应，并在感染后更早恢复，从血液 1 到血液 3 的淋巴细胞浓度以及从血液 3 到血液 4 的血红蛋白浓度和血细胞比容增加幅度更大，但从血液 3 到血液 4 的中性粒细胞浓度低于易感动物和死亡动物 (FDR < 0.05)。响应挑战的 CBC 性状被发现是可遗传的，并且与生长和治疗具有遗传相关性，这可能表明在疾病或商业条件下作为商业系统中的表型发展 CBC 的潜力，作为开发疾病恢复力预测的一部分。
Disease resilience is a valuable trait to help manage infectious diseases in livestock. It is anticipated that improved disease resilience will sustainably increase production efficiency, as resilient animals maintain their performance in the face of infection. The objective of this study was to identify phenotypes related to disease resilience using complete blood count (CBC) data from a wean-to-finish natural disease challenge model, established to mimic the disease pressure caused by many common pathogens at the commercial level of pig production. In total, 2433 F1 crossbred (Landrace x Yorkshire) barrows that went through the natural disease challenge model were classified into four groups (resilient, average, susceptible, and dead) based on their divergent responses in terms of growth and individual treatment. Three sets of blood samples for CBC analysis were drawn at 2-weeks before, and at 2- and 6-weeks after the challenge: Blood 1, Blood 3, and Blood 4 respectively. CBC of Blood 1 taken from healthy pigs before challenge did not show differences between groups. However, resilient animals were found to be primed to initiate a faster adaptive immune response and recover earlier following infection, with greater increases of lymphocyte concentration from Blood 1 to Blood 3 and for hemoglobin concentration and hematocrit from Blood 3 to Blood 4, but a lower neutrophil concentration from Blood 3 to Blood 4 than in susceptible and dead animals (FDR < 0.05). The CBC traits in response to the challenge were found to be heritable and genetically correlated with growth and treatment, which may indicate the potential for developing CBC under disease or commercial conditions as a phenotype in commercial systems as part of developing predictions for disease resilience.