The Antinociceptive Effects of Midazolam on Three Different Types of Nociception in Mice
The Antinociceptive Effects of Midazolam on Three Different Types of Nociception in Mice
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DOI：
10.1254/jphs.08094fp
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发表时间：
2009-01-01
影响因子：
3.5
通讯作者：
Yamada, Yoshitsugu
中科院分区：
文献类型：
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作者：
Chiba, Shunsuke;Nishiyama, Tomoki;Yamada, Yoshitsugu
咪达唑仑全身给药的抗伤害作用仍有争议。本实验观察了其对不同类型伤害性感受的镇痛作用。腹腔内（i. p.）给予四种不同剂量的咪达唑仑（1、3、10和30 mg/kg）。生理盐水用作对照。给药后进行热板试验、尾压试验、醋酸扭体试验、转轮试验和平衡木试验。在热板试验和尾压试验中，腹腔注射咪达唑仑分别在50%有效剂量（艾德（50））或3.46 mg/kg [置信区间（CI），1.99-6.01 mg/kg]和3.52 mg/kg（CI，2.77-4.47 mg/kg）时产生显著的镇痛作用。在醋酸扭体实验中，腹腔注射咪达唑仑也有明显的镇痛作用。在跑轮试验中，没有小鼠在1、3或10 mg/kg的盐水或咪达唑仑后停止跑步，但所有小鼠在腹膜内给予30 mg/kg咪达唑仑后30和45 min停止跑步。在平衡木试验中，腹腔注射生理盐水或1、3和10 mg/kg咪达唑仑后30 min，所有小鼠均能在平衡木上保持90 s，而咪达唑仑30 mg/kg组小鼠均不能。总之，全身给予咪达唑仑对小鼠急性热、急性机械和急性炎症诱导的伤害性感受具有抗伤害性感受作用。咪达唑仑对急性热诱导伤害性感受和机械诱导伤害性感受的抗伤害性感受效力相同。
Antinociceptive effects of systemically administered midazolam remain controversial. The present study was performed to investigate its antinociceptive effects oil different types of nociception in mice. Four different doses of midazolam (1, 3, 10, and 30 mg/kg) were administered intraperitoneally (i.p.). Saline was used as a control. The hot plate test, tail pressure test, acetic acid writhing test, the running wheel test, and the balance beam test were performed following the drug administration. In the hot plate test and tail pressure test, i.p. midazolam produced significant antinociceptive effects with the 50% effective dose (ED(50)) or 3.46 mg/kg [confidence interval (CI), 1.99-6.01 mg/kg] and 3.52 mg/kg (CI, 2.77-4.47 mg/kg), respectively. In the acetic acid writhing test, i.p. midazolam also produced significant antinociceptive effects. In the running wheel test, no mice stopped running after saline or midazolam at 1, 3, or 10 mg/kg, but all mice stopped running 30 and 45 min after i.p. administration of midazolam at 30 mg/kg. In the balance beam test, 30 min after i.p. administration of saline or midazolam at 1, 3, and 10 mg/kg, all mice were able to stay oil the beam for 90s, none of them could with midazolam at 30 mg/kg. In conclusion, systemically administered midazolam had antinociceptive effects on acute thermal, acute mechanical, and acute inflammatory-induced nociception in mice. The antinociceptive potency of midazolam was the same for both acute thermal-induced nociception and mechanical-induced nociception.