Uniform-sized PLA nanoparticles: preparation by premix membrane emulsification.
Uniform-sized PLA nanoparticles: preparation by premix membrane emulsification.
复制标题
DOI：
10.1016/j.ijpharm.2008.03.027
复制
发表时间：
2008-07
影响因子：
5.8
通讯作者：
Q. Wei;Wei Wei-Wei;Bo-Zhang Lai;Lianyan Wang;Yuxia Wang;Z. Su;G. Ma
中科院分区：
文献类型：
--
作者：
Q. Wei;Wei Wei-Wei;Bo-Zhang Lai;Lianyan Wang;Yuxia Wang;Z. Su;G. Ma
纳米粒的粒径大小对药物的释放行为和体内分布有着重要的影响，但对粒径分布窄的可生物降解纳米粒的研究却很少。本文首次采用一种简便的方法，将乳化溶剂去除和预混膜乳化相结合，制备了粒径均匀的纳米粒子。制备粗乳液后，采用超高压预混膜乳化，得到粒径均匀的纳米液滴，进一步固化形成纳米颗粒。选择聚乳酸（PLA）作为模型聚合物。有机溶剂的种类、油相和外水相的体积比、微孔膜的孔径大小以及跨膜压力等因素对聚乳酸纳米粒子的粒径分布具有重要影响。在最佳条件下，聚乳酸纳米粒的变异系数（CV）值可控制在16.9%以下。该方法具有产率高、工艺简单、易于放大等优点。该方法制备的粒径均一的纳米粒在药物释放方面具有很大的应用潜力。
Nanoparticle size is crucial to drug release behavior and biodistribution in vivo, but few studies have been performed on biodegradable nanoparticles with narrow size distribution. In this note, uniform-sized nanoparticles were prepared by a facile method combining emulsion-solvent removal and premix membrane emulsification for the first time. After preparation of coarse emulsions, additional premix membrane emulsification with very high pressure was occupied to achieve uniform-sized nanodroplets, and nanoparticles were formed by further solidification. Polylactide (PLA) was selected as a model polymer. Several factors played key roles to obtain uniform-sized PLA nanoparticles, including type of organic solvent, the volume ratio of oil phase and external water phase, pore size of the microporous membrane and transmembrane pressure. The coefficient of variation (CV) value of PLA nanoparticles could be controlled below 16.9% under an optimum condition. The novel method also has the advantages of high productivity, simplicity and easy scale-up. The uniform-sized nanoparticles prepared by this novel method have great potentials in drug delivery.