Genome-Wide Analysis of the Expression of WRKY Family Genes in Different Developmental Stages of Wild Strawberry (Fragaria vesca) Fruit.
Genome-Wide Analysis of the Expression of WRKY Family Genes in Different Developmental Stages of Wild Strawberry (Fragaria vesca) Fruit.
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野生草莓果实不同发育阶段WRKY家族基因表达的全基因组分析
DOI：
10.1371/journal.pone.0154312
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Xing Y
中科院分区：
文献类型：
--
作者：
Zhou H;Li Y;Zhang Q;Ren S;Shen Y;Qin L;Xing Y
WRKY蛋白在植物衰老、毛状体发生和胚胎形态建成等发育过程中起着重要的调控作用。在草莓中，只有FaWRKY 1（草莓× ananassa）已被鉴定，留下许多WRKY基因有待鉴定和其功能的特点。草莓基因组的基因组序列草图的出版使我们能够在草莓中进行WRKY蛋白的全基因组搜索，并将所鉴定的蛋白质与其在模式植物中的同源物进行比较。从F. vesca基因组基于RNA-seq数据，对该家族的所有成员进行了详细的分析，包括基因分类、注释、系统发育评价、保守基序确定和表达谱分析。此外，利用qRT-PCR技术进一步研究了WRKY基因在草莓果实发育不同阶段的表达模式，为进一步研究这类重要的转录调控因子的比较基因组学和功能奠定基础。
WRKY proteins play important regulatory roles in plant developmental processes such as senescence, trichome initiation and embryo morphogenesis. In strawberry, only FaWRKY1 (Fragaria × ananassa) has been characterized, leaving numerous WRKY genes to be identified and their function characterized. The publication of the draft genome sequence of the strawberry genome allowed us to conduct a genome-wide search for WRKY proteins in Fragaria vesca, and to compare the identified proteins with their homologs in model plants. Fifty-nine FvWRKY genes were identified and annotated from the F. vesca genome. Detailed analysis, including gene classification, annotation, phylogenetic evaluation, conserved motif determination and expression profiling, based on RNA-seq data, were performed on all members of the family. Additionally, the expression patterns of the WRKY genes in different fruit developmental stages were further investigated using qRT-PCR, to provide a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in strawberry.