Ribosomal protein L34 promotes the proliferation, invasion and metastasis of pancreatic cancer cells.
Ribosomal protein L34 promotes the proliferation, invasion and metastasis of pancreatic cancer cells.
复制标题
核糖体蛋白L34促进胰腺癌细胞的增殖、侵袭和转移。
DOI：
10.18632/oncotarget.13269
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发表时间：
2016-12-20
期刊：
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Wei F;Ding L;Wei Z;Zhang Y;Li Y;Qinghua L;Ma Y;Guo L;Lv G;Liu Y
核糖体蛋白（RP）是核糖体的主要成分，参与核糖体的自组装和蛋白质的合成。近年来的研究进展表明，RPs在多种癌症的发生和耐药性中发挥着重要作用。然而，RPL34在胰腺癌（PC）中的表达状态和功能仍不清楚。在这项研究中，我们发现RPL34在PC组织和细胞系中过度表达，这与其启动子甲基化降低相关。敲除RPL34可有效抑制PC细胞的增殖、集落形成、迁移和耐药性，并伴有细胞周期停滞在G2期并诱导细胞凋亡。体内测定表明 RPL34 沉默可抑制 PC 肿瘤的生长和转移。此外，基因表达谱显示 RPL34 沉默会导致 MAPK 和 p53 信号通路的改变。临床上，我们的数据表明 RPL34 表达与 PC 中的肿瘤分期和转移呈正相关。我们发现RPL34是PC中潜在的癌蛋白，RPL34可能是预测预后的有前景的生物标志物和治疗PC的潜在靶点。
Ribosomal proteins (RPs) are the main components of ribosomes and participate in the self-assembly of ribosomes and protein synthesis. Recent advance has shown that RPs play important roles in the tumorigenesis and drug resistance of various cancers. However, the expression status and function of RPL34 in pancreatic cancer (PC) remains unclear. In this study, we find that RPL34 is overexpressed in PC tissues and cell lines, which is correlated with decreased methylation of its promoter. Knockdown of RPL34 effectively suppresses the proliferation, colony formation, migration and drug-resistance of PC cells, which are accompanied by cell cycle arrest at the G2 phase and induction of apoptosis. In vivo assays demonstrate that RPL34 silencing inhibits PC tumor growth and metastasis. Moreover, gene expression profiling revealed that RPL34 silencing results in alteration of the MAPK and p53 signaling pathways. Clinically, our data indicate a positive association of RPL34 expression with tumor stage and metastasis in PCs. We revealed that RPL34 acts as a potential onco-protein in PC, and RPL34 may be a promising biomarker for prognosis prediction and a potential target for the treatment of PC.