Immune characterization of mesenchymal stem cells in human umbilical cord Wharton's jelly and derived cartilage cells
Immune characterization of mesenchymal stem cells in human umbilical cord Wharton's jelly and derived cartilage cells
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DOI：
10.1016/j.cellimm.2012.06.010
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发表时间：
2012-07-01
影响因子：
4.3
通讯作者：
Guo, Quanyi
中科院分区：
文献类型：
--
作者：
Liu, Shuyun;Yuan, Mei;Guo, Quanyi
人脐带间充质干细胞（Mesenchymal stem cells，hWJMSCs）来源广泛、易于获取、增殖能力强、纯度高，是组织工程和细胞治疗的理想种子细胞。这种新生儿干细胞可以从各种胚胎外组织中分离出来，并且似乎比它们的成人对应物更原始，具有更大的多潜能性。本研究通过检测hWJMSCs及其衍生的软骨细胞（hWJMSC-Cs）的主要组织相容性复合物I/I（MHC-I/II）、共刺激分子（CD 40）的表达，探讨hWJMSC-Cs的免疫学特性。CD 80和CD 86）和免疫抑制剂，包括人白细胞抗原G（HLA-G）、吲哚胺-2，3-双加氧酶（1130）和前列腺素E2（PGE 2）。结果表明，hWJMSCs不表达MHC-II和共刺激分子，但中等表达MHC-I，并阳性表达HLA-G、IDO、PGE 2等免疫抑制因子，表明其免疫原性极低，具有诱导宿主免疫耐受微环境的潜力。成软骨分化的hWJMSC（hWJMSC-Cs）的结果与未分化的细胞的结果相似，除了稍微升高的MHC-II和共刺激因子表达。免疫印迹法检测免疫抑制相关分子HGF、VEGF、TGF、IL-10的表达。将hWJMSCs-支架复合物植入家兔和大鼠体内，观察其体内免疫反应，结果显示hWJMSCs在动物体内未引起免疫排斥反应。它们介于成体和胚胎干细胞之间的中间状态使它们成为重新编程为多能状态的理想候选者。需要进一步的研究来阐明hWJMSCs用于软骨和其他组织再生以及基于细胞的治疗的潜力。(C)2012 Elsevier Inc. All rights reserved.
Mesenchymal stem cells derived from human umbilical cord Wharton's jelly (hWJMSCs) became prospective seed cell candidate for tissue engineering and cell-based therapy because of its variety source, easy procurement, robust proliferation, and high purity compared with bone marrow- and adipose-derived MSCs. Such neonatal stem cells can be isolated from a variety of extraembryonic tissues and appear to be more primitive and have greater multi-potentiality than their adult counterparts. In this study, we investigated the immune characters of hWJMSCs and its derived cartilage cells (hWJMSC-Cs) by detecting the expression of major histocompatibility complex I/I(MHC-I/II), costimulatory molecules (CD40. CD80 and CD86) and immune inhibitors including human leukocyte antigen G (HLA-G), indoleamine-2,3-dioxygenase (1130), and prostaglandin E2 (PGE2). We found that hWJMSCs did not express MHC-II and costimulatory molecules, but moderately expressed MHC-I, and positively expressed immune inhibitors as HLA-G, IDO, PGE2, demonstrating their very low immunogenicity and potential to induce immune tolerance microenvironment in hosts. The results of chondrogenic differentiated hWJMSCs(hWJMSC-Cs) are similar to those of undifferentiated cells, except for the slightly elevated MHC-II and costimulators expression. Additionally, we detected cytokine profile of hWJMSCs through cytokine antibody array and verified by western blot the positive expression of immune suppression-related molecules, HGF, VEGF, TGF, and IL-10. Furthermore, to investigate the in vivo immune response of the cells, hWJMSCs-scaffold constructs were implanted into rabbits and rats, and the result showed that hWJMSCs did not elicit immune rejection in the animals. Their intermediate state between adult and embryonic stem cells makes them an ideal candidate for reprogramming to the pluripotent status. Additional studies are necessary to clarify the potential of hWJMSCs to be used in cartilage and other tissue regeneration and cell-based therapies. (C) 2012 Elsevier Inc. All rights reserved.