Isolation and Characterization of Mesenchymal Stromal Cells From Human Degenerated Nucleus Pulposus Comparison With Bone Marrow Mesenchymal Stromal Cells From the Same Subjects
Isolation and Characterization of Mesenchymal Stromal Cells From Human Degenerated Nucleus Pulposus Comparison With Bone Marrow Mesenchymal Stromal Cells From the Same Subjects
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DOI：
10.1097/brs.0b013e3181cb8828
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发表时间：
2010-12-15
期刊：
影响因子：
3
通讯作者：
del Canizo, Maria-Consuelo
中科院分区：
文献类型：
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作者：
Blanco, Juan F.;Graciani, Ignacio F.;del Canizo, Maria-Consuelo
研究设计：从退变的人髓核（NP）中鉴定间充质基质细胞（MSC），并将其与骨髓（BM）间充质基质细胞进行比较。
目的：检测来自同一受试者的髓核和骨髓的间充质基质细胞是否具有相似的生物学特性。
背景资料概述：近期研究提出了治疗椎间盘退变的生物学策略，包括细胞疗法。骨髓间充质基质细胞可能是恢复椎间盘功能的一种有吸引力的方法，并且有证据表明髓核可能含有类间充质基质细胞。
方法：从16例患有退行性椎间盘疾病并接受椎间盘切除术和融合术的患者的退变腰椎髓核以及髂嵴获取组织样本。对从这两种来源分离的间充质基质细胞在扩增时间、免疫表型、分化能力和分子分析方面进行比较。
结果：在所有病例中，均分离并扩增出了髓核间充质基质细胞。它们几乎满足国际细胞治疗学会对间充质基质细胞描述的所有形态学、免疫表型和分化标准，但髓核间充质基质细胞不能分化为脂肪细胞。观察到与来自同一受试者的骨髓间充质基质细胞存在细微差异。
结论：髓核中含有间充质干细胞。这些细胞与骨髓间充质干细胞非常相似，但脂肪分化能力除外。这些发现表明我们可以通过细胞疗法（骨髓间充质基质细胞）以及刺激髓核内源性间充质基质细胞来治疗椎间盘退变。
Study Design. To identify mesenchymal stromal cells (MSC) from degenerate human nucleus pulposus (NP) and compare them with bone marrow (BM) MSC.Objective. To test whether MSC obtained from NP and BM from the same subjects share similar biologic characteristics.Summary of Background Data. Recent studies have proposed biologic strategies for the treatment of intervertebral disc degeneration, including cell therapy. Bone marrow (BM) MSC could be an attractive approach to restore disc function, and there is evidence that NP may contain MSC-like cells.Methods. Tissue samples were obtained from degenerate lumbar NP and from iliac crest of the same 16 patients with degenerative disc diseases, undergoing discectomy and fusion procedures. MSC isolated from both sources were compared regarding their expansion time, immuno-phenotype, differentiation ability, and molecular analysis.Results. In all cases, MSC from NP were isolated and expanded. They fulfil nearly all morphological, inmuno-phenotypical, and differentiation criteria described by the International Society of Cell Therapy for MSC, with the exception that NP-MSC are not able to differentiate into adipocytes. Slight differences were observed with BM-MSC from the same subjects.Conclusion. The NP contains mesenchymal stem cells. These cells were quite similar to mesenchymal stem cells-from BM, with the exception of their adipogenic differentiation ability. These findings suggest that we may treat intervertebral disc degeneration by cell therapy (MSC from BM) and by stimulating endogenous MSC from NP.