Metabolic profiling of the Arabidopsis pkl mutant reveals selective derepression of embryonic traits.
Metabolic profiling of the Arabidopsis pkl mutant reveals selective derepression of embryonic traits.
复制标题
拟南芥 pkl 突变体的代谢谱揭示了胚胎性状的选择性去抑制。
DOI：
10.1007/s00425-004-1254-1
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发表时间：
2004
期刊：
影响因子：
4.3
通讯作者：
Ogas,Joe
中科院分区：
文献类型：
--
作者：
RiderJr,StanleyDean;Hemm,MatthewR;Hostetler,HeatherA;Li,Hui-Chun;Chapple,Clint;Ogas,Joe
胚胎表现出一些独特的分化特征，包括积累一些通常被认为是种子特有的代谢物。PICKLE(PKL)编码一种CHD3-染色质重塑因子，它是抑制拟南芥幼苗胚胎性状所必需的。海因。在突变体中，初生根在萌发后能够表现出许多胚胎性状，被称为泡菜根。为了检测依赖于PKL的抑制胚胎特异性分化途径的广度，我们测定了各种胚胎特异性化合物在泡菜根中积累的程度。我们发现，泡菜根积累了三酰甘油，其脂肪酸组成与种子中发现的类似。主要的种子贮藏蛋白也存在于泡菜根中。除了这两种特性良好的种子储存化合物，我们观察到泡菜根积累植酸，植酸是一种储存的磷酸盐，优先积累在种子中。十字花科成员的种子还积累了各种独特的次生代谢物，包括芥子酸酯和硫代葡萄糖苷。令人惊讶的是，泡菜根中次生代谢物的水平并不暗示胚胎分化状态，但确实揭示了PKL突变导致根次生代谢的实质性变化。综上所述，这些数据表明，PKL负责调节胚胎计划的某些方面，但不是所有方面，因为它与胚胎特异性代谢物的积累有关。
Embryos express several unique differentiation characteristics, including the accumulation of a number of metabolites that are generally considered to be unique to seeds.PICKLE(PKL) codes for a CHD3-chromatin remodeling factor that is necessary for repression of embryonic traits in seedlings ofArabidopsis thaliana(L.) Heynh. Inpklmutants, primary roots are capable of expressing many embryonic traits after germination and are referred to as “pickle roots”. In an attempt to examine the breadth ofPKL-dependent repression of embryo-specific differentiation pathways, we determined the extent to which a variety of embryo-specific compounds accumulate in pickle roots. We found that pickle roots accumulate triacylglycerol with a fatty acid composition that is similar to that found in seeds. The major seed storage proteins are also present in pickle roots. In addition to these two well-characterized seed storage compounds, we observed that pickle roots accumulate phytate, a form of stored phosphate that is preferentially accumulated in seeds. Seeds of members of the Brassicaceae also accumulate a variety of unique secondary metabolites, including sinapate esters and glucosinolates. Surprisingly, the levels of secondary metabolites in pickle roots were not suggestive of an embryonic differentiation state, but did reveal that a mutation inPKLresults in substantial changes in root secondary metabolism. Taken together, these data suggest thatPKLis responsible for regulating some but not all aspects of the embryonic program as it relates to the accumulation of embryo-specific metabolites.