Putative spermine synthases from Thalassiosira pseudonana and Arabidopsis thaliana synthesize thermospermine rather than spermine
Putative spermine synthases from Thalassiosira pseudonana and Arabidopsis thaliana synthesize thermospermine rather than spermine
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DOI：
10.1016/j.febslet.2007.05.074
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发表时间：
2007-06-26
期刊：
影响因子：
3.5
通讯作者：
Sumper, Manfred
中科院分区：
文献类型：
--
作者：
Knott, Juergen M.;Roemer, Piero;Sumper, Manfred
多胺参与了许多基本的细胞过程。常见的多胺有腐胺、亚精胺和精胺。精胺是由脱羧基S-腺苷甲硫氨酸得到的氨丙基残基转移到亚精胺上合成的。热敏剂是精胺的一种异构体，推测是通过类似的机制合成的。然而，最近描述的精胺合成酶中没有一个是作为温度敏合酶的活性被研究的。在这项工作中，从硅藻和拟南芥中推测的精胺合成酶可以被鉴定为温度敏合酶。这些发现可能解释了先前的结果，即拟南芥中两个假定的精胺合成酶基因在基因敲除实验中产生完全不同的表型。很可能，通过序列比较确定的部分推测的精胺合成酶实际上代表了温度敏合酶。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Polyamines are involved in many fundamental cellular processes. Common polyamines are putrescine, spermidine and spermine. Spermine is synthesized by transfer of an ammopropyl residue derived from decarboxylated S-adenosylmethionine to spermidine. Thermospermine is an isomer of spermine and assumed to be synthesized by an analogous mechanism. However, none of the recently described spermine synthases was investigated for their possible activity as thermospermine synthases. In this work, putative spermine synthases from the diatom Thalassiosira pseudonana and from Arabidopsis thaliana could be identified as thermospermine synthases. These findings may explain the previous result that two putative spermine synthase genes in Arabidopsis produce completely different phenotypes in knock-out experiments. Likely, part of putative spermine synthases identifiable by sequence comparisons represents in fact thermospermine synthases. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.