The effect of lipid metabolism disorder on patients with hyperuricemia using Multi-Omics analysis.
The effect of lipid metabolism disorder on patients with hyperuricemia using Multi-Omics analysis.
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DOI：
10.1038/s41598-023-45564-8
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发表时间：
2023-10-24
影响因子：
4.6
通讯作者：
Wang, Xian Min
中科院分区：
文献类型：
--
作者：
Ma, Lili;Wang, Jing;Ma, Li;Ge, Yan;Wang, Xian Min
为探讨新疆高尿酸血症患者脂代谢紊乱对免疫系统的影响，对新疆高尿酸血症患者进行了多组学研究。收集60例健康人和60例高尿酸血症患者的血清。本研究采用液质联用和高效液相色谱分析了不同的脂类代谢物和富集途径。用双抗体夹心法检测免疫因子肿瘤坏死因子-α、白介素6、肉毒碱棕榈酰转移酶-1、转化生长因子-α-1、葡萄糖、乳酸、白介素10、硒蛋白-1的水平，以确定高尿酸血症时脂代谢紊乱的情况。33种差异脂代谢产物在高尿酸血症患者中显著上调。这些脂质代谢产物参与花生四烯酸代谢、甘油磷脂代谢、亚油酸代谢、糖基磷脂酰肌醇(GPI)锚定生物合成和α-亚麻酸代谢途径。IL-10、CPT1、IL-6、sEP1、转化生长因子-β-1、谷氨酸、肿瘤坏死因子-α和LD与甘油磷脂代谢相关。汉族、维吾尔族高尿酸血症患者与健康人比较，血清Cpt1、转化生长因子-β-1、谷氨酸、LD差异有统计学意义(P< <； )。此外，同一种族之间的sEP1、IL-6、转化生长因子-β1、谷氨酸和LD水平有显著差异(P <； 0.0 5)。结果发现，高尿酸血症患者体内33种脂代谢产物存在显著差异，主要涉及5条代谢途径。根据进一步的研究结果，推测CPT1、转化生长因子-β1、SEP1、IL-6、Glu和LD可能通过甘油磷脂途径增加患者的脂肪酸氧化和线粒体氧化磷酸化，降低糖酵解率等途径，改变代谢模式，促进不同的细胞功能，从而影响高尿酸血症患者的疾病进展。
A multiomics study was conducted to investigate how lipid metabolism disorders affect the immune system in Xinjiang patients with hyperuricemia. The serum of 60 healthy individuals and 60 patients with hyperuricemia was collected. This study used LC–MS and HPLC to analyze differential lipid metabolites and enrichment pathways. It measured levels of immune factors tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), carnitine palmitoyltransferase-1 (CPT1), transforming growth factor-β1 (TGF-β1), glucose (Glu), lactic acid (LD), interleukin 10 (IL-10), and selenoprotein 1 (SEP1) using ELISA, as well as to confirm dysregulation of lipid metabolism in hyperuricemia. 33 differential lipid metabolites were significantly upregulated in patients with hyperuricemia. These lipid metabolites were involved in arachidonic acid metabolism, glycerophospholipid metabolism, linoleic acid metabolism, glycosylphosphatidylinositol (GPI)—anchor biosynthesis, and alpha-Linolenic acid metabolism pathways. Moreover, IL-10, CPT1, IL-6, SEP1, TGF-β1, Glu, TNF-α, and LD were associated with glycerophospholipid metabolism. In patients with hyperuricemia of Han and Uyghur nationalities, along with healthy individuals, significant differences in CPT1, TGF-β1, Glu, and LD were demonstrated by ELISA (P < 0.05). Furthermore, the levels of SEP1, IL-6, TGF-β1, Glu, and LD differed considerably between groups of the same ethnicity (P < 0.05). It was found that 33 kinds of lipid metabolites were significantly different in patients with hyperuricemia, which mainly involved 5 metabolic pathways. According to the results of further studies, it is speculated that CPT1, TGF-β1, SEP1, IL-6, Glu and LD may increase fatty acid oxidation and mitochondrial oxidative phosphorylation in patients through glycerophospholipid pathway, reduce the rate of glycolysis, and other pathways to change metabolic patterns, promote different cellular functions, and thus affect the disease progression in patients with hyperuricemia.
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DOI：
10.1152/ajprenal.00104.2006
发表时间：
2007-01-01
影响因子：
4.2
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