Determination of prevalence and economic impact of ovine footrot in central Kashmir India with isolation and molecular characterization of Dichelobacter nodosus
Determination of prevalence and economic impact of ovine footrot in central Kashmir India with isolation and molecular characterization of Dichelobacter nodosus
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DOI：
10.1016/j.anaerobe.2011.02.003
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发表时间：
2011-04-01
期刊：
影响因子：
2.3
通讯作者：
Magray, S. N.
中科院分区：
文献类型：
--
作者：
Rather, M. A.;Wani, S. A.;Magray, S. N.
本研究确定了印度克什米尔中部地区恶臭的流行情况、经济影响，以及迄今尚未开展此类工作的结节双歧杆菌的分离和分子特征。总体而言，克什米尔中部地区有12.54%的足步病患病率，其中斯利那加区最高(15.84%)，布加姆区最低(10.89%)，而甘德巴尔区为13.28%。据估计，狐步舞每年对克什米尔中部的绵羊养殖场产生的总体经济影响达1582万卢比。从自然感染的绵羊足部皮损采集的370份样品中，用聚合酶链式反应(PCR)检测出结节螺旋体阳性200份(54.05%)。其中B群感染132例(66.00%)，E群5例(2.50%)，I群1例(0.50%)，B、E群混合感染53例(26.50%)，B、I群混合感染4例(2.00%)，B、G群混合感染2例(1.00%)，其余3例(1.50%)G群混合感染在印度首次发现。总体而言，B组最常见(97.0%)，其次是E组(30.5%)，而I组(4.0%)和G组(1.0%)最不常见。共分离到265株结节螺旋体，其中B群194株(73.20%)，E群61株(23.01%)，I群8株(3.01%)，G群2株(0.75%)，其中164株(61.88%)具有INTA(整合酶)基因。B群菌株121株(62.37%)，E群36株(59.01%)，G群2株(100%)，I群5株(62.50%)。这表明INTA基因的存在与明胶凝胶法在检测结节螺旋藻毒力方面有很好的相关性。因此，目前的调查建议，根据B和E群的主要流行情况，在制定有效的二价疫苗时纳入B群和E群，以对抗克什米尔中部的狐步病。(C)2011爱思唯尔有限公司。保留所有权利。
The present study determines the prevalence, economic impact of virulent footrot in central Kashmir, India, along with isolation and molecular characterization of Dichelobacter nodosus (D. nodosus) where so far no such work has been carried out. Over all 12.54% prevalence of footrot was recorded in central Kashmir with highest (15.84%) in district Srinagar, and least (10.89%) in district Budgam, while it was 13.28% in district Ganderbal. Overall economic impact of footrot was estimated to the tune of Rs 15.82 million annually to the sheep farming in central Kashmir. Out of 370 samples collected from footrot lesions of naturally infected sheep, 200 (54.05%) detected D. nodosus positive by polymerase chain reaction (PCR). Out of these, 132 (66.00%) samples carried serogroup B of D. nodosus, five (2.50%) serogroup E, one (0.50%) serogroup I, while, 53 (26.50%) had mixed infection of serogroups B and E, four (2.00%) of serogroups B and I, two (1.00%) of serogroups B and G and the remaining three (1.50%) samples harboured the mixed infection of serogroups B, E and I. Serogroup G was detected for the first time in India. Over all serogroup B was most frequent (97.0%) followed by E (30.5%), while serogoups I (4.0%) and G (1.0%) were least prevalent. A total of 265 D.nodosus strains were isolated out of which 194 (73.20%) were typed as serogroup B, 61 (23.01%) as serogroup E. eight (3.01%) as serogroup I and remaining two (0.75%) belonged to serogroup G. Out of 265 D. nodosus isolates, 164 (61.88%) possessed intA (integrase) gene, thus were considered as virulent strains. Serogroup wise intA gene was found in 121(62.37%) isolates of serogroup B, 36 (59.01%) of E, two (100%) of G and five (62.50%) of I. Out of 20 randomly selected isolates subjected to gelatin gel test, 16 isolates with intA gene produced thermostable protease while four isolates without intA gene revealed the production of thermolabile protease. This indicated a good co-relation between presence of intA gene and gelatin gel test in determination of the D. nodosus virulence. Thus the present investigation suggests the incorporation of serogroups B and E. based on their predominant prevalence, in the formulation of an effective bivalent vaccine to combat footrot in central Kashmir. (C) 2011 Elsevier Ltd. All rights reserved.