Plantactinospora solaniradicis sp nov., a novel actinomycete isolated from the root of a tomato plant (Solanum lycopersicum L.)
Plantactinospora solaniradicis sp nov., a novel actinomycete isolated from the root of a tomato plant (Solanum lycopersicum L.)
复制标题
Plantactinospora solaniradicis sp nov.，一种从番茄植物 (Solanum lycopersicum L.) 根中分离出来的新型放线菌
DOI：
10.1007/s10482-017-0943-2
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
Xiang Wensheng
中科院分区：
文献类型：
--
作者：
Li Wenchao;Guo Xiaowei;Shi Linlin;Zhao Junwei;Yan Liangliang;Zhong Xiaotong;Zhang Chen;Chen Yufei;Wang Xiangjing;Xiang Wensheng
从番茄（Solanum lycopersicum L.）采自黑龙江省哈尔滨市。该菌株从基质菌丝上形成表面光滑的单孢子。基于16 S rRNA基因序列的系统发育分析表明，菌株NEAU-FJL 1 T应归属于Plantactinospora属，并与其近邻Plantactinospora soyae NEAU-gxj 3 T形成明显的分支（99.2%的序列相似性）。细胞壁含有meso-二氨基庚二酸，全细胞糖经鉴定为木糖、葡萄糖、阿拉伯糖和半乳糖。主要的甲基萘醌被确定为MK-10（H6）和MK-10（H4）。发现磷脂谱由二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇组成。主要脂肪酸为C15：0、iso-C16：0、anteiso-C17：0、C17：0和iso-C15：0。根据表型特征、系统发育数据和DNA-DNA杂交结果，菌株NEAU-FJL 1 T可与其最接近的菌株区分开来，并被分类为一个新种，命名为Plantactinospora solaniradicissp.十一是建议。模式菌株为NEAU-FJL 1 T（= DSM 100596 T = CGMCC 4.7284T）。
A Gram-positive, non-motile actinomycete, designated strain NEAU-FJL1T, was isolated from tomato root (Solanum lycopersicumL.) collected from Harbin, Heilongjiang province, north China. The strain formed single spores with smooth surfaces from substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-FJL1Tshould be affiliated with the genusPlantactinosporaand forms a distinct branch with its close neighbourPlantactinospora soyaeNEAU-gxj3T(99.2% sequence similarity). The cell wall was found to containmeso-diaminopimelic acid and the whole cell sugars were identified as xylose, glucose, arabinose and galactose. The predominant menaquinones were identified as MK-10(H6) and MK-10(H4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were identified as C15:0, iso-C16:0, anteiso-C17:0, C17:0and iso-C15:0. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, strain NEAU-FJL1Tcan be distinguished from its most closely related strain and classified as a new species, for which the namePlantactinospora solaniradicissp. nov. is proposed. The type strain is NEAU-FJL1T(= DSM 100596T= CGMCC 4.7284T).