Comprehensive description of blood microbiome from healthy donors assessed by 16S targeted metagenomic sequencing
Comprehensive description of blood microbiome from healthy donors assessed by 16S targeted metagenomic sequencing
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DOI：
10.1111/trf.13477
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发表时间：
2016-05-01
期刊：
影响因子：
2.9
通讯作者：
Lelouvier, Benjamin
中科院分区：
文献类型：
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作者：
Paisse, Sandrine;Valle, Carine;Lelouvier, Benjamin
背景：最近的研究表明，健康人的血液并不像以前想象的那样无菌。本研究的目的是提供一个全面的描述的微生物组存在于不同的健康individuals.Study设计和方法的血液馏分：该研究进行了30名健康献血者的法国国家血液采集中心（Etablissement Francais du Sang）。我们已经建立了16S rDNA定量聚合酶链反应测定以及专门设计用于分析血液微生物组的16S靶向宏基因组学测序管道，我们已将其用于全血以及不同的血液组分（血沉棕黄层[BC]、红细胞[RBC]和血浆）。血液中细菌DNA绝大部分位于红细胞（93.74%），红细胞中细菌DNA含量（6.23%）高于血浆（0.03%）。16S DNA的分布对于每个级分是不同的，并且在供体之间分布在相对宽的范围内。在门的水平上，血液成分含有细菌DNA，主要来自变形菌门（超过80%），但也来自放线菌门，厚壁菌门和拟杆菌门。在更深的分类水平上，不同血液组分的细菌谱之间存在显着差异。结论：我们证明了健康血液中存在多样化的微生物组。这种微生物组很可能具有重要的生理作用，并可能与某些输血传播的细菌感染有关。在这方面，可以监测16S细菌DNA的量或微生物组谱，以提高血液供应的安全性。
BACKGROUND: Recent studies have revealed that the blood of healthy humans is not as sterile as previously supposed. The objective of this study was to provide a comprehensive description of the microbiome present in different fractions of the blood of healthy individuals.STUDY DESIGN AND METHODS: The study was conducted in 30 healthy blood donors to the French national blood collection center (Etablissement Francais du Sang). We have set up a 16S rDNA quantitative polymerase chain reaction assay as well as a 16S targeted metagenomics sequencing pipeline specifically designed to analyze the blood microbiome, which we have used on whole blood as well as on different blood fractions (buffy coat [BC], red blood cells [RBCs], and plasma).RESULTS: Most of the blood bacterial DNA is located in the BC (93.74%), and RBCs contain more bacterial DNA (6.23%) than the plasma (0.03%). The distribution of 16S DNA is different for each fraction and spreads over a relatively broad range among donors. At the phylum level, blood fractions contain bacterial DNA mostly from the Proteobacteria phylum (more than 80%) but also from Actinobacteria, Firmicutes, and Bacteroidetes. At deeper taxonomic levels, there are striking differences between the bacterial profiles of the different blood fractions.CONCLUSION: We demonstrate that a diversified microbiome exists in healthy blood. This microbiome has most likely an important physiologic role and could be implicated in certain transfusion-transmitted bacterial infections. In this regard, the amount of 16S bacterial DNA or the microbiome profile could be monitored to improve the safety of the blood supply.