Wet ashing in biological samples in a microwave oven under pressure using poly(tetrafluoroethylene) vessels.
Wet ashing in biological samples in a microwave oven under pressure using poly(tetrafluoroethylene) vessels.
复制标题
使用聚四氟乙烯容器在微波炉中在压力下对生物样品进行湿灰化。
作者：
P. Aysola;P. Anderson;C. Langford
戈萨奇描述了与生物样品中有机物氧化有关的问题。这一步骤必须先于原子光谱法测定痕量金属。在热板上用酸处理通常需要1-2小时。使用微波炉是加速这一过程的一个有吸引力的可能性。Koirtyohann et al.和Barrett et al.通过增加排气口来改进微波炉。纳德卡尼利用一个未经改造的烤箱，使用一个耐热玻璃真空干燥器作为加压容器。他们报告了大量的Cu（26%）和Pb（20%）损失。Matts等人尝试过聚碳酸酯加压容器，但这种塑料很快就变得不透明且易碎。作者重新评估了使用加压容器的未经修改的微波炉的前景。他们发现耐热玻璃容器在玻璃中迅速吸收热量。用特氟龙TFA牌氟碳树脂代替聚碳酸酯，发现其具有优越的化学性能和机械性能。他们现在报告了使用未经修改的商业烤箱的60年代压力容器程序。
Gorsuch has described the problems associated with the oxidation of organic matter in biological samples. This is a step that must precede trace metal determination by atomic spectroscopy. Treatment with acid on a hot plate typically requires 1-2 h. The use of a microwave oven is an attractive possibility for acceleration of the process. Koirtyohann et al. and Barrett et al. modified microwave ovens by adding an exhaust port. Nadkarni exploited an unmodified oven by using a Pyrex vacuum desiccator as a pressurizable vessel. They report significant losses of Cu (26%) and Pb (20%). Matts et al. tried polycarbonate pressurizable vessels, but the plastic quickly became opaque and brittle. The authors have reevaluated the prospects for use of an unmodified microwave oven with pressurized vessels. They found that Pyrex vessels gain heat in the glass quickly. The authors substituted Teflon TFA brand fluorocarbon resin for polycarbonate and find it has superior chemical and mechanical properties. They now report a 60-s pressure vessel procedure using an unmodified commercial oven.