Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development
Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development
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DOI：
10.1016/s0014-5793(02)02904-6
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发表时间：
2002-07-17
期刊：
影响因子：
3.5
通讯作者：
Rabbitts, P
中科院分区：
文献类型：
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作者：
Clark, K;Hammond, E;Rabbitts, P
果蝇轴突引导受体环路的哺乳动物同源物在广泛的组织中表达。在这里，我们证明了dut1/Robo1基因的选择性剪接导致了两个具有不同信号肽的mRNA转录本，它们在小鼠胚胎发育过程中有不同的表达。由于定向缺失的dut1/Robo1基因小鼠存在肺病理异常，采用免疫组织化学方法检测了dut1/Robo1基因在肺发育过程中的细胞表达模式。在胚胎17.5天时，肺组织中有广泛和弥漫的dut1/Robo1的表达，但在新生和成年小鼠中，它们的表达越来越局限于支气管上皮。(C)2002年，由Elsevier Science B.V.代表欧洲生化学会联合会出版。
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.