A Possible Mechanism of Autoimmune-Mediated infertility in Women with Endometriosis
A Possible Mechanism of Autoimmune-Mediated infertility in Women with Endometriosis
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DOI：
10.1111/j.1600-0897.2010.00956.x
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发表时间：
2011-08-01
影响因子：
3.6
通讯作者：
Matsuura, Eiji
中科院分区：
文献类型：
--
作者：
Inagaki, Junko;Hao, Lin;Matsuura, Eiji
问题子宫内膜异位症被认为是一种自身免疫性疾病，因为存在多种针对子宫内膜或卵巢抗原的自身抗体。本研究的目的是表征抗层粘连蛋白-111自身抗体在子宫内膜异位症不孕患者中的结合特异性，并探讨这些自身抗体是否影响体外胚胎发育。研究方法：采用覆盖层粘连蛋白-α 1链整个G结构域的重叠合成肽进行ELISA分析，在子宫内膜异位症不孕患者中进行（n = 45）。小鼠囊胚培养在培养基中含有纯化的IgG从一个抗体阳性血清层粘连蛋白-111-coated dish.ResultsAnti-laminin-111自身抗体针对层粘连蛋白-α 1链G结构域中的几个特定的生物功能肽序列。测试的IgG显着抑制体外滋养层outgrowth.ConclusionAnti-laminin-111自身抗体的程度可能有主要的致病作用，对早期生殖功能衰竭，包括糖尿病相关不孕症。
ProblemEndometriosis has been proposed to be an autoimmune disease because of the presence of a variety of autoantibodies specific for endometrial or ovarian antigens. The object of the present study is to characterize binding specificity of anti-laminin-111 autoantibodies in infertile patients with endometriosis and to investigate whether these autoantibodies affect the in vitro embryo development.Method of studyAn ELISA analysis using overlapping synthesized peptides that covered the entire G domain of laminin-alpha 1 chain was performed in infertile patients with endometriosis (n = 45). Mouse blastocysts were cultured in media containing the purified IgG from one antibody-positive serum on laminin-111-coated dishes.ResultsAnti-laminin-111 autoantibodies were directed to several particular biologically functional peptide sequences in laminin-alpha 1 chain G domain. The tested IgG significantly inhibited the extent of in vitro trophoblast outgrowth.ConclusionAnti-laminin-111 autoantibodies may have major pathogenic roles on early reproductive failure including endometriosis-associated infertility.