Ki-ras mutations and prognosis in colorectal cancer
Ki-ras mutations and prognosis in colorectal cancer
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DOI：
10.1016/s0959-8049(97)10111-3
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发表时间：
1998-03-01
影响因子：
8.4
通讯作者：
Borresen-Dale, AL
中科院分区：
文献类型：
--
作者：
Kressner, U;Bjorheim, J;Borresen-Dale, AL
为了评估Ki-ras基因突变与肿瘤分期、肿瘤分化程度和生存时间的可能关系，我们对191例结直肠腺癌进行了研究，这些腺癌均来自连续随访的患者，中位随访时间为6年。使用时间温度梯度凝胶电泳（TTGE）对切除的全交叉肿瘤样本进行Ki-ras密码子12和13突变筛查。Ki-ras基因突变检出率为32%。在21个样本中观察到的最常见的突变是从GGT到GAT，将密码子12中的甘氨酸改变为天冬氨酸。该研究未显示Ki-ras突变与Dukes分期或肿瘤分化之间存在任何关联。Ki-ras突变患者的生存时间（中位数50个月）略短于无突变患者（中位数59个月），但差异无统计学意义。结果表明，Ki-ras基因突变没有相关的预后意义，在这个队列的结直肠癌患者。(C)1998爱思唯尔科技有限公司版权所有。
A total of 191 colorectal adenocarcinomas, obtained from consecutive patients with a median followup of 6 years, were studied in order to evaluate the possible association of Ki-ras mutations with tumour stage, tumour differentiation and survival time. Resected full-cross tumour samples were screened for Ki-ras mutations in codons 12 and 13 using temporal temperature gradient gel electrophoresis (TTGE). Ki-ras mutations were detected in 62 (32%) of the samples. The most frequent mutation, observed in 21 samples, was from GGT to GAT changing glycine to aspartic acid in codon 12. The study did not show any association between Ki-ras mutations and Dukes' stage or tumour differentiation. Patients with Ki-ras mutations had a marginally shorter survival time (median 50 months) compared with patients without (median 59 months), but the difference was not statistically significant. The results indicate that Ki-ras gene mutations have no relevant prognostic importance in this cohort of colorectal cancer patients. (C) 1998 Elsevier Science Ltd. All rights reserved.