Complex Genetics Control Natural Variation in Arabidopsis thaliana Resistance to Botrytis cinerea
Complex Genetics Control Natural Variation in Arabidopsis thaliana Resistance to Botrytis cinerea
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DOI：
10.1534/genetics.108.091439
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发表时间：
2008-12-01
期刊：
影响因子：
3.3
通讯作者：
Kliebenstein, Daniel J.
中科院分区：
文献类型：
--
作者：
Rowe, Heather C.;Kliebenstein, Daniel J.
植物的遗传结构。对微生物病原体的防御可能受到病原体生活方式的影响。虽然植物与生物营养病原体的相互作用通常由遵循经典孟德尔遗传的大效抗性基因的作用控制，但我们的研究表明，植物对坏死性病原体灰葡萄孢菌的防御主要是数量和遗传复杂的。对坏死性病原体的定量抗性研究很少使用大型植物图谱群体来剖析抗性的遗传结构。利用拟南芥的大型结构定位群体，我们确定了影响植物对绿僵菌反应的数量性状位点。通过坏死病变油叶的扩张和抗菌化合物camalexin的积累来测量。通过对多个灰芽孢杆菌分离株的检测，我们在该群体中鉴定出23个独立的QTL，这些QTL的分离特异性从单个分离株鉴定到控制对所有分离株的抗性不等。我们确定了一个集合。对病原菌感染控制camalexin积累的QTL研究已确定的抗性QTL似乎在涉及三个或更多位点的上位性网络中起作用。多位点连接的检测表明，特定信号或反应网络的自然变异可能控制拟南芥- b。在这个种群中灰霉病相互作用。
The genetic architecture of plant. defense against microbial pathogens may be influenced by pathogen lifestyle. While plant interactions with biotrophic pathogens are frequently controlled by the action of large-effect resistance genes that follow classic Mendelian inheritance, our study suggests that plant defense against the necrotrophic pathogen Botrytis cinerea is primarily quantitative and genetically complex. Few studies of quantitative resistance to necrotrophic pathogens have used large plant mapping populations to dissect the genetic structure of resistance. Using a large structured mapping population of Arabidopsis thaliana, we identified quantitative trait loci influencing plant response to B. cinerea., measured as expansion of necrotic lesions oil leaves and accumulation of the antimicrobial compound camalexin. Testing multiple B. cinerea isolates, we identified 23 separate QTL in this population, ranging ill isolate-specificity from being identified with a single isolate to Controlling resistance against, all isolates tested. We identified a set. of QTL controlling accumulation of camalexin in response to pathogen infection that. largely colocalized with lesion QTL The identified resistance QTL appear to function in epistatic networks involving three or more loci. Detection of multilocus connections suggests that natural variation in specific signaling or response networks may control A. thaliana-B. cinerea interaction in this population.