Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats.
Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF rats.
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DOI：
10.1186/1476-511x-3-24
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发表时间：
2004-11-09
影响因子：
4.5
通讯作者：
Yanagita, Teruyoshi
中科院分区：
文献类型：
--
作者：
Arao, Keisuke;Wang, Yu-Ming;Yanagita, Teruyoshi
共轭脂肪酸是具有共轭双键的多不饱和脂肪酸的位置异构体和几何异构体的总称，因其潜在的有益生物学效应而引起人们的广泛关注。在本研究中，石榴籽油富含石榴酸（9顺，11反，13顺-共轭亚麻酸; 9 c，11 t，13 c-CLNA）对肥胖，高脂血症大冢Long-Evans德岛脂肪（OLETF）大鼠脂质代谢的影响进行了研究。饲养2周后，OLETF大鼠与其祖先LETO大鼠相比表现出肥胖和高脂血症。在OLETF大鼠中，与补充10%红花油的饮食（对照饮食）相比，补充9%红花油和1%石榴籽油的饮食（9 c，11 t，13 c-CLNA饮食）不影响腹部白色脂肪组织重量和血脂水平。然而，在OLETF大鼠中，9 c，11 t，13 c-CLNA饮食显著降低肝脏三酰甘油的积累。与脂肪酸合成和脂肪酸β-氧化相关的肝酶活性不受9 c，11 t，13 c-CLNA饮食的影响。单不饱和脂肪酸（MUFA），脂肪酸的主要储存形式，在血清三酰甘油的水平显着高于肥胖，高血脂的OLETF大鼠瘦LETO大鼠。此外，9 c，11 t，13 c-CLNA饮食显著降低OLETF大鼠中的MUFA水平。这是第一项研究表明，9 c，11 t，13 c-CLNA抑制δ-9去饱和在体内，我们认为，减轻肝脏三酰甘油积累的9 c，11 t，13 c-CLNA饮食是，至少部分，可归因于抑制δ-9去饱和在OLETF大鼠。
Conjugated fatty acid, the general term of positional and geometric isomers of polyunsaturated fatty acids with conjugated double bonds, has attracted considerable attention because of its potentially beneficial biological effects. In the present study, dietary effect of pomegranate seed oil rich in punicic acid (9cis, 11trans, 13cis-conjugated linolenic acid; 9c, 11t, 13c-CLNA) on lipid metabolism was investigated in obese, hyperlipidemic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. After 2 weeks feeding period, OLETF rats revealed obesity and hyperlipidemia compared with their progenitor LETO rats. Feeding of the diet supplemented with 9% safflower oil and 1% pomegranate seed oil (9c, 11t, 13c-CLNA diet) did not affect abdominal white adipose tissue weights and serum lipid levels compared with the diet supplemented with 10% safflower oil (control diet) in OLETF rats. However, the accumulated hepatic triacylglycerol was markedly decreased by 9c, 11t, 13c-CLNA diet in OLETF rats. Activities of hepatic enzymes related to fatty acid synthesis and fatty acid beta-oxidation were not altered by 9c, 11t, 13c-CLNA diet. Levels of monounsaturated fatty acid (MUFA), major storage form of fatty acid, in serum triacylglycerol were markedly higher in obese, hyperlipidemic OLETF rats than in lean LETO rats. In addition, 9c, 11t, 13c-CLNA diet significantly decreased MUFA levels in OLETF rats. This is the first study showing that 9c, 11t, 13c-CLNA suppresses delta-9 desaturation in vivo, and we suggest that the alleviation of hepatic triacylglycerol accumulation by 9c, 11t, 13c-CLNA diet was, at least in part, attributable to the suppression of delta-9 desaturation in OLETF rats.