VEGF deficit is involved in endothelium dysfunction in preeclampsia
VEGF deficit is involved in endothelium dysfunction in preeclampsia
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DOI：
10.1007/s11596-010-0359-y
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发表时间：
2010-06
期刊：
影响因子：
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通讯作者：
Qiong Zhou;Haiyi Liu;F. Qiao;Yuanyuan Wu;Jingjing Xu
中科院分区：
文献类型：
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作者：
Qiong Zhou;Haiyi Liu;F. Qiao;Yuanyuan Wu;Jingjing Xu
本研究检测了血管内皮生长因子（VEGF）的表达与子痫前期内皮功能障碍的关系，VEGF是一种促进血管生成的因子。采用免疫组化、实时逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附试验（ELISA）分别检测30例子痫前期患者和30例血压正常孕妇胎盘和外周血中VEGF蛋白和mRNA水平。VEGF在人脐静脉内皮细胞（HUVECs）中的表达被小干扰rna （sirna）阻断。通过测定穿过HUVEC单层的BSA的荧光强度来测定HUVEC的单层屏障功能。MTT法和硝酸还原酶法分别检测细胞增殖和细胞分泌一氧化氮（NO）水平。结果表明，VEGF在胎盘组织的合胞滋养细胞和血管、毛细血管内皮细胞中均有表达。虽然子痫前期患者胎盘组织中VEGF mRNA的表达仍然较高，但子痫前期患者血清中VEGF水平与正常妊娠相比明显降低。此外，VEGF缺乏可导致内皮细胞功能障碍，并可保护内皮细胞免受损伤。由此可见，VEGF的缺乏可能导致内皮细胞功能障碍，从而导致子痫前期的发生和发展。
This study examined the association of expression of vascular endothelial growth factor (VEGF), a promoter of angiogenesis, with endothelium dysfunction in preeclampsia. The level of VEGF protein and mRNA in the placenta and peripheral blood samples of 30 preeclampsia patients and 30 normotensive pregnant women was measured by immunohistochemistry, real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. VEGF expression in the human umbilical vein endothelial cells (HUVECs) was blocked by small interfering RNAs (siRNAs). The monolayer barrier function of HUVECs was determined by measuring the fluorescence intensity of BSA that crossed the HUVEC monolayers. The cell proliferation and cell-secreted nitric oxide (NO) level were detected by MTT method and nitrate reductase assay, respectively. The results showed that VEGF was expressed in the syncytiotrophoblasts and endothelial cells of vessels and capillaries in the placenta tissue. The serum level of VEGF in the preeclampsia patients was significantly decreased as compared with that in normal pregnant subjects, although VEGF mRNA expression in the placenta tissue of preeclampsia patients remained still high. Moreover, VEGF deficit could lead to endothelium cell dysfunction, and the administration of VEGF could protect endothelium cells from injury. It was concluded that lack of VEGF contributes to endothelium dysfunction, which may lead to the occurrence and development of preeclampsia.