Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation
Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation
复制标题
去唾液酸化促红细胞生成素对冻融犬卵巢异种移植的有益作用
DOI：
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发表时间：
2008
影响因子：
3.1
通讯作者：
H. Saitoh
中科院分区：
文献类型：
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作者：
Hiroshi Suzuki;Takako Ishijima;Saori Maruyama;Yoshiko Yanagimoto Ueta;Y. Abe;H. Saitoh
目的卵巢冷冻保存后移植的主要缺点是移植后大量卵泡丢失。因此，我们观察了促红细胞生成素（EPO）和去氧化EPO对冻融犬卵巢异种移植的影响。方法采用NOD-SCID小鼠，观察EPO和去乙酰化EPO对冻融犬卵巢移植后卵泡的保护作用和促存活作用。将冷冻解冻的狗卵巢组织与400 U/kg的EPO或亚洲EPO放入卵巢囊。结果移植后4周切除卵巢，行组织学检查。EPO组的早期原发卵泡存活率为15.2%，亚洲EPO组为157.6%，而未治疗组为10.1%。结论本实验结果表明，亚麻黄EPO可有效提高冷冻卵巢移植后卵泡的存活率。
PurposeThe main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost after transplantation. Thus, effects of erythropoietin (EPO) and desialylated EPO administration on the frozen-thawed canine ovarian xenotransplantation were examined.MethodsThe protective and survival-promoting effects of EPO and desialylated EPO on the follicles of frozen-thawed canine ovaries after transplantation were examined using NOD-SCID mice. Frozen-thawed dog ovarian tissue with 400 U/kg of EPO or asialo EPO was placed into the ovarian bursa.ResultsAt 4 weeks after the transplantation, the ovaries were removed and subjected to histological examination. The survival rate of early primary follicles was 15.2% in the EPO group and 157.6% in the asialo EPO group, in contrast to 10.1% in the untreated group.ConclusionsThese results demonstrate that administration of asialo EPO could be effectively used to enhance the survival of the follicles of transplanted cryopreserved ovaries.