Sensitivity and reversibility of Ca-dependent inhibition of the (Na+ + K+)-ATPase of human red blood cells.
Sensitivity and reversibility of Ca-dependent inhibition of the (Na+ + K+)-ATPase of human red blood cells.
复制标题
人红细胞 (Na K )-ATPase 的 Ca 依赖性抑制的敏感性和可逆性。
DOI：
10.1016/0005-2736(85)90243-3
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Polasek,PM
中科院分区：
文献类型：
--
作者：
Yingst,DR;Polasek,PM
部分纯化的人红细胞溶血物提取液可使(Na++K+)-ATPase对钙抑制的敏感性提高30倍。溶血组分将50%抑制所需的游离钙浓度从30μM降低到约30 Mm。1μM。有或无溶血组分对(Na++K+)-ATPase的钙依赖性抑制是完全可逆的。溶血组分还能刺激Ca~(2+)-ATPase，增加其对钙的亲和力。在溶血组分存在的情况下，抑制(Na++K+)-ATPase 50%的游离钙浓度与半最大刺激钙-ATPase的浓度相似。沸腾组分破坏了其对(Na++K+)-ATPase的作用，但不削弱其对钙-ATPase的刺激。
The sensitivity of the (Na++ K+)-ATPase to inhibition by Ca was increased 30-fold by a partially purified extract of human red cell hemolysate. The hemolysate fraction reduced the concentration of free Ca required for 50% inhibition from 30 μM to approx. 1 μM. Ca-dependent inhibition of the (Na++ K+)-ATPase in the presence and absence of the hemolysate fraction was completely reversible. The hemolysate fraction also stimulated the Ca2+-ATPase and increased its affinity for Ca. In the presence of the hemolysate fraction, the concentration of free Ca that inhibited the (Na++ K+)-ATPase by 50% was similar to that which half-maximally stimulated the Ca2+-ATPase. Boiling the fraction destroyed its effect on the (Na++ K+)-ATPase, but did not impair its stimulation of the Ca2+-ATPase.