Tertiary butyl hydroperoxide induced oxidative damage in mice erythrocytes: Protection by taurine.
Tertiary butyl hydroperoxide induced oxidative damage in mice erythrocytes: Protection by taurine.
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DOI：
10.1016/j.pathophys.2012.05.001
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发表时间：
2012-04-01
期刊：
影响因子：
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通讯作者：
Sil, Parames C
中科院分区：
文献类型：
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作者：
Roy, Anandita;Sil, Parames C
本研究旨在探讨牛磺酸对叔丁基过氧化氢（TBHP）诱导的小鼠红细胞氧化应激的保护作用。红细胞单独用TBHP或用牛磺酸处理，然后TBHP暴露。TBHP诱导的氧化应激增加高铁血红蛋白的形成，脂质过氧化和蛋白质羰基化的红细胞。然而，同样的暴露会耗尽细胞GSH含量，改变抗氧化酶和高铁血红蛋白还原酶的活性，降低Ca（+）和Na（+）/K（+）ATP酶的活性和细胞内ATP水平。牛磺酸转运抑制剂β-丙氨酸处理的红细胞在TBHP暴露时表现出增加的磷脂酰丝氨酸外化和ROS形成，并且牛磺酸不能逆转该效应。TBHP暴露增加细胞内钙和上调钙蛋白酶的水平。然而，牛磺酸的管理可以防止TBHP诱导的氧化失衡。红细胞的电子显微镜照片显示改变的形态与棘细胞的数量增加。牛磺酸处理可恢复红细胞抗氧化酶和代谢产物的正常水平。结果表明，TBHP给药引起的红细胞氧化损伤主要通过膜稳定作用被牛磺酸阻止。
The present study was undertaken to investigate the protective role of taurine, against t-butyl hydroperoxide (TBHP) induced oxidative stress in murine erythrocytes. Erythrocytes were treated either with TBHP alone or with taurine, followed by TBHP exposure. TBHP-induced oxidative stress increased methemoglobin formation, lipid peroxidation and protein carbonylation in erythrocytes. The same exposure, however, depleted cellular GSH content and altered the activities of the antioxidant enzymes as well as of methemoglobin reductase; reduced activities of Ca(+) and Na(+)/K(+) ATPase and intracellular ATP levels. Taurine transport inhibitor, beta-alanine, treated erythrocytes showed increased phosphatidylserine externalization and ROS formation on TBHP exposure and taurine could not revert the effect. TBHP exposure increased intracellular calcium and upregulated the level of calpain. Administration of taurine could, however, prevent the TBHP induced oxidative imbalance. Electron micrographs of erythrocytes showed changed morphology with an increase in the number of echinocytes. Taurine treatment could restore the normal levels of the antioxidant enzymes and metabolites of the erythrocytes. Results suggest that the oxidative insult introduced in erythrocytes by TBHP administration is prevented by taurine mainly via membrane stabilization.