Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow.
Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow.
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DOI:
10.1016/j.ymeth.2008.10.022
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发表时间:
2009-03
期刊:
影响因子:
4.8
通讯作者:
Quinones, Edwin
中科院分区:
文献类型:
--
作者:
Leuba, Sanford H.;Wheeler, Travis B.;Cheng, Chao-Min;LeDuc, Philip R.;Fernandez-Sierra, Monica;Quinones, Edwin
关键词:
Here we describe experiments which employ magnetic tweezers and or microfluidics to manipulate single DNA molecules. We describe the use of magnetic tweezers coupled to an inverted microscope as well as the use of a magnetic tweezers setup with an upright microscope. Using a chamber prepared via soft lithography, we also describe a microfluidic device for the manipulation of individual DNA molecules. Finally, we present some past successful examples of using these approaches to elucidate unique information about protein-nucleic acid interactions.
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