INTERACTIONS OF THYROID-HORMONE, GROWTH-HORMONE, AND HIGH-CARBOHYDRATE, FAT-FREE DIET IN REGULATING SEVERAL RAT-LIVER MESSENGER RIBONUCLEIC-ACID SPECIES
INTERACTIONS OF THYROID-HORMONE, GROWTH-HORMONE, AND HIGH-CARBOHYDRATE, FAT-FREE DIET IN REGULATING SEVERAL RAT-LIVER MESSENGER RIBONUCLEIC-ACID SPECIES
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DOI：
10.1021/bi00270a031
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
TOWLE, HC
中科院分区：
文献类型：
--
作者：
LIAW, C;SEELIG, S;TOWLE, HC
本实验观察了甲状腺激素、生长激素和高碳水化合物、无脂饮食对大鼠肝细胞内mRNA水平的多效性调节作用。在mRNA依赖性网织红细胞裂解物系统中翻译从适当处理的动物中分离的总肝含poly（A）RNA。35 S标记的翻译产物的二维凝胶电泳允许分离apprx。200种不同的mRNA编码产物使用计算机化视频密度测定法定量任何单个产品在总人群中的相对比例。先前的工作已经表明，对甲状腺功能减退大鼠施用三碘甲状腺原氨酸（T3）会导致α的特异性改变。8%的mRNA种类通过该技术检测。这些T3反应mRNA种类的一个子集通过其对垂体生长激素产生的作用而间接受到T3的影响。给予甲状腺功能减退大鼠生长激素导致8种不同mRNA水平的特异性改变。生长激素使4种mRNA的相对水平降低，4种mRNA的相对水平升高。因此，这种可能与膜受体结合的激素可以在翻译前水平起作用，以增加和减弱几种特定的mRNA序列。对于这些mRNA种类中的2种，T3和生长激素的联合作用对于维持正常水平是必要的。此外，将大鼠从标准实验室食物转换为高碳水化合物和无脂肪的饮食导致10种不同mRNA编码产物的水平发生变化。有趣的是，除了一种以外，所有这些mRNA种类也受到动物甲状腺状态的影响。因此，由T3和高碳水化合物、无脂肪饮食调节的mRNA种类之间存在高度重叠。大多数T3-应答mRNA种类似乎在复杂的多因子途径中受到调节。
Pleiotropic effects of thyroid hormone, growth hormone and high carbohydrate, fat-free diet in regulating the intracellular levels of rat liver mRNA were examined. Total hepatic poly(A)-containing RNA, isolated from appropriately treated animals, was translated in the mRNA-dependent reticulocyte lysate system. Two-dimensional gel electrophoresis of the 35S-labeled translational products allowed separation of .apprx. 200 different mRNA-encoded products. Computerized video densitometry was utilized to quantitate the relative proportion of any individual product in the total population. Previous work has shown that administration of triiodothyronine (T3) to hypothyroid rats leads to specific alterations in .apprx. 8% of the mRNA species detected by this technique. A subset of these T3-responsive mRNA species is indirectly affected by T3 through its action on pituitary production of growth hormone. Administration of growth hormone to hypothyroid rats leads to specific alterations in the levels of 8 different mRNA species. The relative levels of 4 mRNA species are diminished, and those of 4 mRNA species are increased by growth hormone. Thus, this hormone which presumably binds to a membrane receptor can act at a pretranslational level to augment and attenuate several specific mRNA sequences. For 2 of these mRNA species, the combined action of T3 and growth hormone is necessary for maintenance of normal levels. In addition, switching rats from standard laboratory chow to a diet high in carbohydrate and fat free resulted in changes in the levels of 10 different mRNA-encoded products. Interestingly, all but one of these mRNA species was also influenced by the thyroidal status of the animal. Thus, a high degree of overlap exists between mRNA species regulated by T3 and a high carbohydrate, fat-free diet. Most T3-responsive mRNA species appear to be regulated in a complex multifactorial pathway.