Intracellular pH distribution in foraminifera determined by the fluorescent probe HPTS
Intracellular pH distribution in foraminifera determined by the fluorescent probe HPTS
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DOI：
10.4319/lom.2008.6.610
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Kitazato, H.
中科院分区：
文献类型：
--
作者：
de Nooijer, L. J.;Toyofuku, T.;Kitazato, H.
化石有孔虫是重建过去海洋环境的极好工具，因为其碳酸钙的化学性质反映了海水的许多物理和化学参数。然而，有孔虫方解石中的微量元素/钙的比例在物种之间和标本内各不相同，通常偏离无机沉淀方解石中发现的比例。生物控制CaCO 3沉淀是最有可能进行修改的pH值在网站上的钙化或从母液中除去Mg 2+。在这项研究中，我们展示了应用无毒的荧光探针8-羟基芘-1，3，6-三磺酸（HPTS）在有孔虫细胞内的pH值分析。在所有分析的类群，高pH值囊泡的大小为3-5 μ m的细胞中发现，通常在较高的密度向最终室。在粟粒种Quinqueloculina costata中发现了pH值最高（>= 8.5）的囊泡：透明种显示pH值为8.0-8.5的囊泡。这些结果表明，在钙化过程中的pH值，从而对微量元素分区有孔虫控制提供基本的见解的应用HPTS的潜力。
Fossil foraminifera are excellent tools to reconstruct past marine environments since the chemistry of their calcium carbonate reflects many physical and chemical parameters of seawater. However, trace elemental/calcium ratios in foraminiferal calcite vary between species and within specimens, usually deviating from ratios found in inorganically precipitated calcite. Biological control on CaCO3 precipitation is most likely to proceed by modification of the pH at the site of calcification or by removal of Mg2+ from the mother solution. In this study, we show the application of the nontoxic fluorescent probe 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) in analyzing foraminiferal intracellular pH. In all analyzed taxa, high-pH vesicles with a size of 3-5 mu m were found in the cell, commonly in higher densities toward the ultimate chamber. Vesicles with the highest pH (>= 8.5) were found in the miliolid species Quinqueloculina costata: hyaline species displayed vesicles with a pH of 8.0-8.5. These results show the potential of the application of HPTS in providing fundamental insights in foraminiferal control on the pH during calcification and thereby on trace elemental partitioning.