Isolation and characterization of polymorphic microsatellites in the perennial herb Euphorbia kansui using paired-end Illumina shotgun sequencing
Isolation and characterization of polymorphic microsatellites in the perennial herb Euphorbia kansui using paired-end Illumina shotgun sequencing
复制标题
使用双端 Illumina 鸟枪法测序对多年生草本植物甘遂中的多态性微卫星进行分离和表征
DOI：
10.1007/s12686-014-0274-9
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发表时间：
2014-12-01
影响因子：
1.1
通讯作者：
Zhao, Gui-Fang
中科院分区：
文献类型：
--
作者：
Yan, Xiao-Hao;Fang, Min-Feng;Zhao, Gui-Fang
甘遂是我国特有的一种濒危多年生草本植物，对其种群遗传学的研究对于制定有效的保护和管理策略至关重要。本研究开发了12个新的微卫星标记。kansui使用Illumina测序技术。等位基因数在2 ~ 11个之间，平均每个位点4.1个等位基因。期望杂合度（HE）为0.0950 ~ 0.7875，观察杂合度（HO）为0.0500 ~ 0.9500，平均杂合度分别为0.4050和0.4083。在错误发现率控制程序之后，仅在单个位点（Ek 1073）中检测到显著偏离Hardy-Weinberg平衡。这些微卫星标记信息丰富，可用于该濒危植物的遗传分析。
Euphorbia kansui is a threatened perennial herb endemic to China, and a good knowledge of its population genetics would be essential to the formulation of effective conservation and management strategies. In this study, 12 novel microsatellite markers were developed for E. kansui using Illumina sequencing technology. The number of alleles varied between 2 and 11, with an average of 4.1 alleles per locus. The expected (HE) and observed heterozygosity (HO) ranged from 0.0950 to 0.7875 and from 0.0500 to 0.9500, with an average of 0.4050 and 0.4083, respectively. Following the false discovery rate controlling procedure, significant deviations from Hardy–Weinberg equilibrium was only detected in a single locus (Ek1073). These informative microsatellite markers can be readily used for genetic analyses of this threatened plant.