DISTRIBUTION, CLASSIFICATION, AND DEVELOPMENT OF DROSOPHILA GLIAL-CELLS IN THE LATE EMBRYONIC AND EARLY LARVAL VENTRAL NERVE CORD
DISTRIBUTION, CLASSIFICATION, AND DEVELOPMENT OF DROSOPHILA GLIAL-CELLS IN THE LATE EMBRYONIC AND EARLY LARVAL VENTRAL NERVE CORD
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DOI：
10.1007/bf02179499
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发表时间：
1995-05-01
期刊：
影响因子：
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通讯作者：
TECHNAU, GM
中科院分区：
文献类型：
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作者：
ITO, K;URBAN, J;TECHNAU, GM
为了便于果蝇胶质细胞发育的研究，我们对果蝇腹神经索中的胶质细胞进行了详细的描述。对胶质细胞特异性表达进行GAL 4增强子捕获筛选。使用UAS-lacZ和UAS-kinesin-lacZ作为报告构建体，我们描述了刚孵化后的第一龄幼虫完全分化的腹神经索中所识别的胶质细胞的分布和形态。利用计算机重建神经胶质网络的三维结构。使用在胚胎发育后期具有一致GAL 4表达的菌株，我们追溯了所鉴定细胞的发育，以提供胚胎期16的神经胶质图。在胚胎和早期幼虫中，我们通常每个腹部神经节鉴定出60（54-64）个胶质细胞。它们分为三个类别下的六个亚型：表面相关胶质细胞（16-18个神经束膜下胶质细胞和6-8个通道胶质细胞）、皮质相关胶质细胞（6-8个细胞体胶质细胞）和神经纤毛相关胶质细胞（8-10个神经根胶质细胞、14-16个界面胶质细胞和3-4个中线胶质细胞）。建议的神经胶质分类系统进行了讨论，与以前的昆虫神经胶质分类。
To facilitate the investigation of glial development in Drosophila, we present a detailed description of the Drosophila glial cells in the ventral nerve cord. A GAL4 enhancer-trap screen for glial-specific expression was performed. Using UAS-lacZ and UAS-kinesin-lacZ as reporter constructs, we describe the distribution and morphology of the identified glial cells in the fully differentiated ventral nerve cord of first-instar larvae just after hatching. The three-dimensional structure of the glial network was reconstructed using a computer. Using the strains with consistent GAL4 expression during late embryogenesis, we traced back the development of the identified cells to provide a glial map at embryonic stage 16. We identify typically 60 (54-64) glial cells per abdominal neuromere both in embryos and early larvae. They are divided into six subtypes under three categories: surface-associated glia (16-18 subperineurial glial cells and 6-8 channel glial cells), cortex-associated glia (6-8 cell body glial cells), and neuropile-associated glia (8-10 nerve root glial cells, 14-16 interface glial cells, and 3-4 midline glial cells). The proposed glial classification system is discussed in comparison with previous insect glial classifications.