Decreased Expression of MicroRNA-375 in Nonsmall Cell Lung Cancer and its Clinical Significance
Decreased Expression of MicroRNA-375 in Nonsmall Cell Lung Cancer and its Clinical Significance
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DOI：
10.1177/030006051204000505
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发表时间：
2012-09-01
影响因子：
1.6
通讯作者：
Hu, C.
中科院分区：
文献类型：
--
作者：
Li, Y.;Jiang, Q.;Hu, C.
目的：新出现的证据表明，在许多类型的人类恶性肿瘤中，异常microRNA-375（miR-375）表达与肿瘤发生相关。这项前瞻性研究描述了miR-375对非小细胞肺癌（NSCLC）发生和进展的贡献。方法：使用实时逆转录-聚合酶链反应前瞻性地检测96对NSCLC组织和邻近非癌组织（距离癌组织>2 cm）样本中的miR-375水平。还探讨了miR-375水平与临床病理特征之间的关系。结果：与匹配的非癌组织样本相比，miR-375在89%（85/96）的NSCLC样本中下调。miR-375的降低与晚期疾病分期和淋巴结转移显著相关。单变量和多变量考克斯比例风险回归分析显示，miR-375低表达是NSCLC总生存期的不利预后因素。结论：本研究提示miR-375是一种新的NSCLC预后指标，可能成为NSCLC诊断和基因治疗的潜在靶点。
OBJECTIVE: Emerging evidence has shown the association of aberrant microRNA-375 (miR-375) expression with tumourigenesis in many types of human malignancy. This prospective study characterized the contribution of miR-375 to the initiation and progression of nonsmall cell lung cancer (NSCLC). METHODS: The real-time reverse transcription-polymerase chain reaction was used to examine miR-375 levels prospectively in 96 pairs of samples of NSCLC tissue and adjacent noncancerous tissue (>2 cm from cancer tissue). The relationship between miR-375 levels and clinicopathological features was also explored. RESULTS: MiR-375 was downregulated in 89% (85/96) of NSCLC samples compared with matched noncancerous tissue samples. Decreased miR-375 correlated significantly with advanced disease stage and lymphatic metastasis. Univariate and multivariate Cox proportional hazard regression analyses revealed that underexpression of miR-375 was an unfavourable prognostic factor for overall survival in NSCLC. CONCLUSIONS: This study suggested that miR-375 is a novel prognostic indicator in NSCLC and might be a potential target for diagnosis and gene therapy.