Susceptibility to hyperosmotic stress-induced phosphatidylserine exposure increases during red blood cell storage
Susceptibility to hyperosmotic stress-induced phosphatidylserine exposure increases during red blood cell storage
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DOI：
10.1111/j.1537-2995.2010.02929.x
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发表时间：
2011-05-01
期刊：
影响因子：
2.9
通讯作者：
Novotny, Vera M. J.
中科院分区：
文献类型：
--
作者：
Bosman, Giel J. C. G. M.;Cluitmans, Judith C. A.;Novotny, Vera M. J.
背景技术背景：在输血前储存红细胞（RBC）期间，RBC经历了一系列结构和功能变化，包括暴露于磷脂酰丝氨酸（PS），这是一种有效的清除信号。据推测，在血库储存过程中，应激诱导PS暴露的敏感性增加，从而使相当一部分的红细胞易于快速去除transmitting.Study设计和方法：红细胞进行处理和储存后，标准的荷兰血库程序。每周采集样品，持续长达6周，并暴露于各种应激条件，如高渗休克和能量消耗。这些处理对PS暴露的影响通过膜联蛋白V结合的流式细胞术分析来测量。同样的分析进行了红细胞已被分离，根据密度使用不连续Percoll gradient.Results：在存储过程中血库条件下，红细胞变得越来越容易损失的磷脂不对称诱导的高渗休克和能量消耗。特别是较高密度的红细胞，具有较小的体积和增加的HbA1c含量，是典型的老年红细胞，变得越来越容易与storage time.CONCLUSIONS：在存储过程中，红细胞开发的敏感性增加应力诱导的磷脂不对称性损失，特别是与老化表型。这种增加的易感性可能是输血后前24小时内相当一部分RBC迅速消失的原因。
BACKGROUND: During storage of red blood cell (RBCs) before transfusion, RBCs undergo a series of structural and functional changes that include the exposure of phosphatidylserine (PS), a potent removal signal. It was postulated that, during blood bank storage, the susceptibility to stress-induced PS exposure increases, thereby rendering a considerable fraction of the RBCs susceptible to rapid removal after transfusion.STUDY DESIGN AND METHODS: RBCs were processed and stored following standard Dutch blood bank procedures. Samples were taken every week for up to 6 weeks and exposed to various stress conditions, such as hyperosmotic shock and energy depletion. The effect of these treatments on PS exposure was measured by flow cytometric analysis of annexin V binding. The same analyses were performed on RBCs that had been separated according to density using discontinuous Percoll gradients.RESULTS: During storage under blood bank conditions, RBCs become increasingly susceptible to loss of phospholipid asymmetry induced by hyperosmotic shock and energy depletion. Especially the RBCs of higher densities, that have a smaller volume and an increased HbA1c content as is typical of aged RBCs, become increasingly susceptible with storage time.CONCLUSIONS: During storage, RBCs develop an increased susceptibility to stress-induced loss of phospholipid asymmetry that is especially associated with an aging phenotype. This increased susceptibility may be responsible for the rapid disappearance of a considerable fraction of the RBCs during the first 24 hours after transfusion.