Digital data storage on DNA tape using CRISPR base editors.
Digital data storage on DNA tape using CRISPR base editors.
复制标题
使用 CRISPR 碱基编辑器将数字数据存储在 DNA 磁带上。
DOI：
10.1101/2023.02.07.527074
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Zadegan,Reza
中科院分区：
文献类型：
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作者：
Sadremomtaz,Afsaneh;Glass,RobertF;Guerrero,JorgeEduardo;LaJeunesse,DennisR;Josephs,EricA;Zadegan,Reza
While the archival digital memory industry approaches its physical limits, the demand is significantly increasing, therefore alternatives emerge. Recent efforts have demonstrated DNA’s enormous potential as a digital storage medium with superior information durability, capacity, and energy consumption. However, the majority of the proposed systems require on-demand de-novo DNA synthesis techniques that produce a large amount of toxic waste and therefore are not industrially scalable and environmentally friendly. Inspired by the architecture of semiconductor memory devices and recent developments in gene editing, we created a molecular digital data storage system called “DNA Mutational Overwriting Storage” (DMOS) that stores information by leveraging combinatorial, addressable, orthogonal, and independent in vitro CRISPR base-editing reactions to write data on a blank pool of greenly synthesized DNA tapes. As a proof of concept, this work illustrates writing and accurately reading of both a bitmap representation of our school’s logo and the title of this study on the DNA tapes.
