Genetic transformation in Haemophilus parainfluenzae clinical isolates
Genetic transformation in Haemophilus parainfluenzae clinical isolates
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DOI：
10.1007/s002849900349
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发表时间：
1998-08-01
影响因子：
2.6
通讯作者：
Dove, MG
中科院分区：
文献类型：
--
作者：
Gromkova, RC;Mottalini, TC;Dove, MG
研究了从呼吸道疾病患者中回收的副流感嗜血杆菌分离株通过同基因DNA进行遗传转化的能力。两个染色体标记，链霉素抗性和萘啶酸抗性，在H。三种生物型的副流感病毒受体。最有效的转化是生物型II，其次是生物型I，而生物型III是不可转化的。缺乏转化并不是因为DNA的供体活性差，而是因为细胞不能形成感受态。在液体培养基中形成团块的菌株是不可转化的。由于可转化生物型II是世界范围内流行的生物型之一，可以推测DNA转化可能在H.副流感
Haemophilus parainfluenzae isolates recovered from patients with respiratory diseases were studied for their ability to undergo genetic transformation by isogenic DNA. Two chromosomal markers, streptomycin resistance and nalidixic acid resistance, were tested for transformation efficiencies in H. parainfluenzae recipients from three biotypes. Most efficient in transformation was biotype II, followed by biotype I, while biotype III was nontransformable. Lack of transformation was not owing to poor donor activity of DNA, but to inability of the cells to develop competence. Strains that formed clumps in liquid media were nontransformable. Since the transformable biotype II is one of the prevalent biotypes world wide, one can speculate that DNA transformation probably plays a major role in the spread of drug resistance in H. parainfluenzae.